Enantioselective liquid-liquid extraction of tryptophan enantiomers by a recyclable aqueous biphasic system based on stimuli-responsive polymers
Enantioselective liquid-liquid extraction of tryptophan enantiomers by a recyclable aqueous biphasic system based on stimuli-responsive polymers
复制标题
基于刺激响应聚合物的可回收水性双相系统对色氨酸对映体进行对映选择性液-液萃取
DOI：
10.1016/j.chroma.2021.462532
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发表时间：
2021
影响因子：
4.1
通讯作者：
Zhijian Tan
中科院分区：
文献类型：
--
作者：
Shaoping Ma;Fenfang Li;Zhijian Tan
传统的对映选择性液液萃取（ELLE）一直使用疏水性有机溶剂/水双相体系。近年来，水性双相系统（ABS）被认为是《ELLE》中使用的一种有前景的方法。在本工作中，由温度响应性聚合物聚（MAH-β-CD-co-NIPAAm）（PN-CD）和pH响应性聚合物聚（AA-DMAEMA-BMA）（PADB）组成的可回收ABS用于色氨酸对映体的对映分离。聚合物PN-CD不仅作为相形成组分，而且作为手性选择剂，可以通过改变温度进行回收。聚合物PADB可以作为相形成组分，也可以通过调节pH值进行回收。研究了PN-CD/PADBABS 的相行为。研究了该手性分离过程的影响参数，包括聚合物浓度、初始色氨酸浓度、萃取温度和系统pH。在最佳条件下一步萃取得到的最大分离因子(α)为1.42。同时，L-色氨酸（L-Trp）和D-色氨酸（D-Trp）的分配系数分别为2.79和1.96。本研究利用 ELLE 开发了一种绿色且可持续的对映体分离策略。
The hydrophobic organic solvents/water biphasic system had been always used in the traditional enantioselective liquid-liquid extraction (ELLE). In recent years, aqueous biphasic systems (ABSs) are considered as a promising method used in the ELLE. In the present work, a recyclable ABS composed of a temperature-responsive polymer poly(MAH-β-CD-co-NIPAAm) (PN-CD) and a pH-responsive polymer poly(AA-DMAEMA-BMA) (PADB) was employed in the enantioseparation of tryptophan enantiomers. The polymer PN-CDacted as not only the phase-forming component but also the chiral selector, which can be recycled by changing the temperature. The polymer PADBcan be used as the phase-forming component, which can also be recycled by adjusting the pH. The phase behaviors of this PN-CD/PADBABS had been studied. The influencing parameters were studied for this chiral separation process, including the polymer concentration, initial tryptophan concentration, extraction temperature, and system pH. The maximum separation factor (α) of 1.42 was obtained by one-step extraction under the optimal conditions. Meanwhile, the distribution coefficients ofL-tryptophan (L-Trp) andD-tryptophan (D-Trp) were 2.79 and 1.96, respectively. This study develops a green and sustainable strategy for enantioseparation by using the ELLE.