Growth state-dependent phenotypes of adult hepatocytes in primary monolayer culture.
Growth state-dependent phenotypes of adult hepatocytes in primary monolayer culture.
复制标题
原代单层培养物中成体肝细胞的生长状态依赖性表型。
DOI：
10.1073/pnas.75.4.1834
复制
发表时间：
1978
影响因子：
11.1
通讯作者：
I. Arias
中科院分区：
文献类型：
--
作者：
H. Leffert;T. Moran;S. Sell;H. Skelly;K. Ibsen;M. Mueller;I. Arias
一个增殖能力的成年大鼠肝细胞单层系统已分析其生长周期中的组织特异性功能。在早期滞后期观察到高水平的成人（L型）形式的丙酮酸激酶（EC 2.7.1.40）和谷胱甘肽S-转移酶B（“配体”，EC 2.5.1.18）;它们在对数期显著下降，并在稳定期重新出现。相比之下，胎儿（K型）形式的丙酮酸激酶和甲胎蛋白的生产水平升高出现后，增殖开始，这种模式略有减少，在稳定期的成人表型恢复。白蛋白的产生贯穿整个生长周期。这些体外结果模拟了在完整动物肝增殖转变期间观察到的结果，因此，构成了原代培养中正常上皮细胞的发育程序。
A proliferation-competent adult rat liver cell monolayer system has been analyzed for tissue-specific functions during its growth cycle. High levels of the adult (L type) form of pyruvate kinase (EC 2.7.1.40) and glutathione S-transferase B ("ligand," EC 2.5.1.18) are observed during the early lag phase; they decline markedly during the logarithmic phase and reappear during the stationary phase. By contrast, elevated levels of the fetal (K type) form of pyruvate kinase and alpha1-fetoprotein production appear only after proliferation begins; this pattern diminishes slightly during stationary phase as the adult phenotype is restored. Albumin production continues throughout the entire growth cycle. These in vitro findings simulate those observed during hepatoproliferative transitions in the intact animal and, as such, constitute a developmental program for normal epithelial cells in primary culture.