Paeoniflorin augments systemic Candida albicans infection through inhibiting Th1 and Th17 cell expression in a mouse model
Paeoniflorin augments systemic Candida albicans infection through inhibiting Th1 and Th17 cell expression in a mouse model
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芍药苷通过抑制小鼠模型中的 Th1 和 Th17 细胞表达增强全身白色念珠菌感染
DOI：
10.1016/j.intimp.2018.03.001
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发表时间：
2018-07-01
影响因子：
5.6
通讯作者：
Liu, Weida
中科院分区：
文献类型：
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作者：
Kong, Xue;Leng, Dongni;Liu, Weida
芍药苷（PF）是一种具有双重免疫调节作用的中草药，在我国临床上应用广泛。以前的研究发现PF抑制白色念珠菌（C.然而，PF是否在体内发挥抗真菌作用仍然是未知的。在这项研究中，我们试图检查PF单独或与抗真菌剂，氟康唑（FCZ），使用小鼠模型的系统性念珠菌病的影响。结果显示，与单独感染组和FCZ治疗组相比，PF单独或PF + FCZ治疗组小鼠的存活时间分别降低（8.20 ± 1.75 vs 10.40 ± 2.50天，P < 0.05; 24.60 ± 6.55 vs 29.00 ± 3.16天，P < 0.05）。与单独感染组或FCZ治疗组相比，PF单独治疗组和PF + FCZ治疗组小鼠肾脏中的真菌负荷增加。此外，发现PF和PF + FCZ治疗组显示血清干扰素γ（IFN-γ）、白细胞介素（IL）-17和IL-22水平显著降低，血清IL-4水平升高; PF对肿瘤坏死因子α（TNF-α）的产生没有影响。PF单独或与FCZ联合使用可降低Th 1（IFN-γ（+）CD 4（+））和Th 17细胞（IL-17（+）CD 4（+））的增殖，增加Th 2细胞（IL-4（+）CD 4（+））的表达。这些结果表明，PF处理可能是有害的主机响应系统C。小鼠白色念珠菌感染。因此，在真菌感染患者中临床使用PF可能需要谨慎。
Paeonifiorin (PF), a Chinese herbal medicine, has been widely used in clinical practice in China because of its dual immunoregulatory effects. A previous study found that PF inhibited the biofihn formation of Candida albicans (C. albicans) in vitro; however, whether PF plays an antifungal role in vivo is still unexplored. In this study, we sought to examine the effect of PF alone or in combination with an antifungal agent, fluconazole (FCZ), using a mouse model of systemic candidiasis. The results showed that the survival time of mice treated with PF alone or PF + FCZ decreased compared with the Infected alone and FCZ treated groups, respectively (8.20 +/- 1.75 vs 10.40 +/- 2.50 days, P < 0.05; 24.60 +/- 6.55 vs 29.00 +/- 3.16 days, P < 0.05). The fungal burden in the kidney of mice increased in the PF alone and PF + FCZ treated groups compared with the Infected alone or FCZ treated group. Furthermore, it was found that the PF and PF + FCZ treated groups showed significantly decreased levels of serum interferon gamma (IFN-gamma), interleukin (IL)-17, and IL-22, and an increased level of serum IL-4; PF had no effect on the production of tumor necrosis factor alpha (TNF-alpha). PF alone or in combination with FCZ decreased the proliferation of Thl (IFN-gamma(+) CD4(+)) and Th17 cells (IL-17(+) CD4(+)) and increased the expression of Th2 cells (IL-4(+) CD4(+)). These results suggested that PF treatment could be detrimental to the host response to systemic C. albicans infection in mice. Thus, caution might be required for clinical use of PF in patients with fungal infection.