Vitreous levels of pigment epithelium-derived factor and vascular endothelial growth factor: Implications for ocular angiogenesis
Vitreous levels of pigment epithelium-derived factor and vascular endothelial growth factor: Implications for ocular angiogenesis
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DOI：
10.1016/j.ajo.2003.11.015
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发表时间：
2004-04-01
影响因子：
4.2
通讯作者：
Zack, DJ
中科院分区：
文献类型：
--
作者：
Duh, EJ;Yang, HS;Zack, DJ
目的：色素上皮衍生因子(PEDF)已在多种动物模型中被证实抑制眼血管生成。在这项研究中，我们试图测定有和没有眼部新生血管疾病患者玻璃体中PEDF和血管内皮生长因子(VEGF)的水平。设计：对接受各种新生血管和非新生血管疾病的眼内手术患者进行病例对照研究。方法：收集65例65只眼的玻璃体样本，包括无新生血管疾病(n=24)、脉络膜新生血管(n=9)、活动性增殖性糖尿病视网膜病变(n=16)和非活动性增殖性糖尿病视网膜病变(n=16)。结果：参照组和脉络膜新生血管组玻璃体液中的血管内皮生长因子水平均低于可检测水平。PDR活动期和非活动期的血管内皮生长因子水平均显著升高，且活动期PDR组高于非活动期PDR组。与对照组和脉络膜新生血管组相比，活动期PDR患者的PEDF水平高于对照组和脉络膜新生血管组。结论：无论有无眼部新生血管，玻璃体中均有高水平的PEDF表达，但活动期PDR患者的PEDF水平明显高于脉络膜新生血管或非新生血管疾病患者。尽管这些结果不能排除内源性PEDF有助于调节眼球新生血管的可能性，但它们不支持缺血诱导的PEDF下调作为这种调节的机制。(C)2004，由Elsevier Inc.保留所有权利。
PURPOSE: Pigment epithelium,derived factor (PEDF) has been demonstrated to suppress ocular angiogenesis in several animal models. In this study, we sought to measure the levels of PEDF and vascular endothelial growth factor (VEGF) in the vitreous of patients with and without ocular neovascular disorders.DESIGN: Case-control study of patients undergoing intraocular surgery for a variety of neovascular and nonneovascular conditions.METHODS: Vitreous samples were collected from 65 eyes of 65 patients with no neovascular disorder (n = 24), choroidal neovascularization (n = 9), active proliferative diabetic retinopathy (n = 16), and inactive proliferative diabetic retinopathy (n = 16). The levels of VEGF and PEDF in these vitreous samples were determined by enzyme,linked immunosorbent assay.RESULTS: The VEGF levels were at or below the level of detectability in the reference and choroidal neovascularization groups. The VEGF levels were significantly elevated in both the active and inactive PDR groups, and significantly higher in the active PDR group as compared with the inactive PDR group. The PEDF levels, which were present at relatively high concentrations in all groups, were higher in patients with active PDR compared with the control and choroidal neovascularization groups. 0CONCLUSIONS: High levels of immunoreactive PEDF are present in the vitreous of individuals with or without ocular neovascularization, but PEDF levels are significantly higher in patients with active PDR compared with patients with choroidal neovascularization or nonneovascular retinal diseases. Although these results do not preclude the possibility that endogenous PEDF helps to modulate ocular neovascularization, they do not support ischemia,induced downregulation of PEDF as a mechanism for such modulation. (C) 2004 by Elsevier Inc. All rights reserved.