A Gain-of-Function Screen Identifying Genes Required for Growth and Pattern Formation of the Drosophila melanogaster Wing
A Gain-of-Function Screen Identifying Genes Required for Growth and Pattern Formation of the Drosophila melanogaster Wing
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DOI：
10.1534/genetics.109.107748
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发表时间：
2009-11-01
期刊：
影响因子：
3.3
通讯作者：
de Celis, Jose F.
中科院分区：
文献类型：
--
作者：
Cruz, Cristina;Glavic, Alvaro;de Celis, Jose F.
果蝇翅膀是用于分析器官大小、形状和图案形成的遗传控制的模型系统。翅膀的形成涉及细胞生长、增殖、图案形成和分化等多种过程。这些发育过程受到遗传控制，许多参与翅膀发育特定方面的基因已经被表征。在这项工作中，我们的目标是识别调节翅膀生长和图案的新基因。为此，我们进行了功能获得筛选，生成新型 P-UAS（上游激活序列）插入，允许强制基因表达。我们生产了 3340 个新颖的 P-UAS 插入，并分离了 300 个，它们与仅在假定的翼片中央区域表达的 Ga14 驱动器相结合，导致各种机翼表型。这些 P-UAS 插入位点的定位使其能够识别导致功能获得表型的基因。我们发现这些表型的一部分与异位基因表达域中细胞死亡的诱导有关。最后，我们对筛选中确定的基因进行了初步表征，该基因的功能是 L5 纵静脉发育所必需的。
The Drosophila melanogaster wing is a model system for analyzing the genetic control of organ size, shape, and pattern formation. The formation of the wing involves a variety of processes, such as cell growth, proliferation, pattern formation, and differentiation. These developmental processes are under genetic control, and many genes participating in specific aspects of wing development have already being characterized. In this work, we aim to identify novel genes regulating wing growth and patterning. To this end, we have carried Out a gain-of-function screen generating novel P-UAS (upstream activating sequences) insertions allowing forced gene expression. We produced 3340 novel P-UAS insertions and isolated 300 that cause a variety of wing phenotypes in combination with a Ga14 driver expressed exclusively in the central domain of the presumptive wing blade. The mapping of these P-UAS insertion sites allowed its to identify the gene that causes the gain-of-function phenotypes. We show that a fraction of these phenotypes are related to the induction of cell death in the domain of ectopic gene expression. Finally, we present a preliminary characterization of a gene identified in the screen, the function of which is required for the development of the L5 longitudinal vein.