Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography
Histologic correlation of pig retina radial stratification with ultrahigh-resolution optical coherence tomography
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DOI：
10.1167/iovs.02-0654
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发表时间：
2003-04-01
影响因子：
4.4
通讯作者：
Drexler, W
中科院分区：
文献类型：
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作者：
Gloesmann, M;Hermann, B;Drexler, W
目的。将猪视网膜的超高分辨率光学相干断层扫描（OCT）横断面图像与组织学进行比较，以评估超高分辨率OCT在增强视网膜内和视网膜下结构可视化方面的潜力。从家猪体外后眼杯制剂中获得轴向分辨率为1.4 μ m ×横向分辨率为3 μ m的超高分辨率OCT图像。冷冻切片与OCT断层图精确对齐，使用主要血管作为定位标记，用甲酚紫反染色或不染色，用差示干涉对比显微镜检查。组织学切片的显微照片线性缩放以校正组织收缩，并与OCT断层图进行比较。在视网膜近端，超高分辨率OCT信号带直接对应主要视网膜层。对于所使用的波长区域（类似于800 nm），轴突层（神经纤维层、内外丛状层）比细胞体层（神经节细胞层、内核层、外核层）反射更强。在远端视网膜，可见光感受器层的亚结构，如内外节之间的界面，并分辨视网膜色素上皮、绒毛膜毛细层和浅层脉络膜。此外，采用超高分辨率oct监测视网膜脱离事件的时间顺序。体外眼超分辨率OCT成像显示，视网膜形态具有前所未有的细节。OCT信号模式对视网膜亚结构的特定分配为提高对具有高度临床相关性的眼内OCT断层扫描的解释奠定了基础。
PURPOSE. To compare ultrahigh-resolution optical coherence tomography (OCT) cross-sectional images of the pig retina with histology, to evaluate the potential of ultrahigh-resolution OCT for enhanced visualization of intra- and subretinal structures.METHODS. Ultrabigh-resolution OCT images were acquired with 1.4-mum axial x 3-mum transverse resolution from in vitro posterior eyecup preparations of the domestic pig. Frozen sections were obtained in precise alignment with OCT tomograms, by using major blood vessels as orientation markers and were counterstained with cresyl violet or unstained and examined by differential interference contrast microscopy. Micrographs from histologic sections were linearly scaled to correct for tissue shrinkage and compared with OCT tomograms.RESULTS. In the proximal retina, ultrahigh-resolution OCT signal bands directly corresponded to the main retinal layers. For the wavelength region used (similar to800 nm), axodendritic layers (nerve fiber layer, inner and outer plexiform layers) were more reflective than cell body layers (ganglion cell layer, inner nuclear layer, outer nuclear layer). In the distal retina, substructures of the photoreceptor layer such as the interface between inner and outer segments were visualized, and the retinal pigment epithelium, the choriocapillaris, and superficial choroid layers were resolved. In addition, the time sequence of a retinal detachment event was monitored by ultrahigh-resolution OCT.CONCLUSIONS. In vitro ophthalmic ultrahigh-resolution OCT imaging reveals, retinal morphology with unprecedented detail. The specific assignment of OCT signal patterns to retinal substructures provides a basis for improved interpretation of in vivo ophthalmic OCT tomograms of high clinical relevance.