Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study
Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study
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血液微生物组与 2 型糖尿病之间的关联：一项巢式病例对照研究
DOI：
10.1002/jcla.22842
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发表时间：
2019-05-01
影响因子：
2.7
通讯作者：
Dong, Chen
中科院分区：
文献类型：
--
作者：
Qiu, Jing;Zhou, Hui;Dong, Chen
虽然最近的研究表明肠道微生物群失调与2型糖尿病（T2DM）的发病显著相关，但关于血液微生物群在T2DM发病中的作用的信息却很少。方法从135例前瞻性队列研究中选择50例T2DM病例和100例匹配的非T2DM对照。利用诊断前血液样本的细菌16S核糖体RNA （16S rRNA）基因测序对血液微生物组的组成进行了表征。在Illumina MiSeq仪器上使用MiSeq Reagent Kit PE300 v3试剂盒对扩增子进行归一化、合并和测序。结果T2DM患者和非T2DM对照组分别获得3 000 391和6 244 227个高质量序列。两组血液微生物组的平均多样性（Simpson、Chao1和Shannon指数）在基线时无显著差异。在属水平上，水藻菌、黄单胞菌和伪心菌的丰度较低，而放线菌、Alishewanella、Sediminibacterium和Pseudoclavibacter在T2DM患者中的丰度高于非T2DM对照组。结果显示，血液中携带拟杆菌属的参与者与T2DM发展风险降低显著相关，74%对88%（调整后OR: 0.367, 95% CI: 0.151-0.894）。然而，携带Sediminibacterium属的参与者患T2DM的风险增加，调整OR （95% CI）为14.098（1.358,146.330）。结论血液微生物组可能在T2DM发病过程中起病因学作用。这些发现将有助于制定基于微生物群的T2DM预防和控制策略。
Background Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. Methods Fifty incident T2DM cases and 100 matched non-T2DM controls were selected from a prospective cohort study of "135." The composition of the blood microbiome was characterized using bacterial 16S ribosomal RNA (16S rRNA) gene sequencing from pre-diagnostic blood sample. The amplicons were normalized, pooled, and sequenced on the Illumina MiSeq instrument using a MiSeq Reagent Kit PE300 v3 kit. Results Totally, 3 000 391 and 6 244 227 high-quality sequences were obtained from T2DM patients and non-T2DM controls, respectively. The mean diversity of the blood microbiome (Simpson, Chao1 and Shannon indices) was not different between two groups at baseline. At genus level, the Aquabacterium, Xanthomonas, and Pseudonocardia were presented with lower abundance, while Actinotalea, Alishewanella, Sediminibacterium, and Pseudoclavibacter were presented with higher abundance among T2DM cases compared to those in non-T2DM controls. As the results shown, participants carried the genus Bacteroides in blood were significantly associated with a decreased risk for T2DM development, with 74% vs 88% (adjusted OR: 0.367, 95% CI: 0.151-0.894). However, participants carried the genus Sediminibacterium have an increased risk for T2DM, with adjusted OR (95% CI) being 14.098 (1.358, 146.330). Conclusions Blood microbiome may play an etiology role in the development of T2DM. These findings would be useful to develop microbiota-based strategies for T2DM prevention and control.