Transport of dsRNA into cells by the transmembrane protein SID-1
Transport of dsRNA into cells by the transmembrane protein SID-1
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DOI：
10.1126/science.1087117
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发表时间：
2003-09-12
期刊：
影响因子：
56.9
通讯作者：
Hunter, CP
中科院分区：
文献类型：
--
作者：
Feinberg, EH;Hunter, CP
RNA干扰（RNAi）在植物和线虫中系统性传播，以沉默远离起始位点的基因表达。我们以前确定了一个基因，sid-1，必需的系统性，但不是细胞自主RNAi秀丽隐杆线虫。在这里，我们证明，SID-1是一个多跨膜蛋白，敏感果蝇细胞浸泡RNAi的效力，是依赖于双链RNA（dsRNA）的长度。进一步的分析表明，SID-1能够使细胞被动摄取dsRNA。这些数据表明，系统性RNAi在C. elegans涉及SID-1介导的dsRNA的细胞间转运。
RNA interference (RNAi) spreads systemically in plants and nematodes to silence gene expression distant from the site of initiation. We previously identified a gene, sid-1, essential for systemic but not cell-autonomous RNAi in Caenorhabditis elegans. Here, we demonstrate that SID-1 is a multispan transmembrane protein that sensitizes Drosophila cells to soaking RNAi with a potency that is dependent on double-stranded RNA ( dsRNA) length. Further analyses revealed that SID-1 enables passive cellular uptake of dsRNA. These data indicate that systemic RNAi in C. elegans involves SID-1-mediated intercellular transport of dsRNA.