Experimental and morphological studies of the noradrenaline innervations in the nucleus tractus spinalis nervi trigemini of the rat with special reference to their fine structures
Experimental and morphological studies of the noradrenaline innervations in the nucleus tractus spinalis nervi trigemini of the rat with special reference to their fine structures
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大鼠三叉神经棘束核去甲肾上腺素神经支配的实验和形态学研究，特别是其精细结构
DOI：
10.1016/0006-8993(81)90099-8
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
N. Shimizu
中科院分区：
文献类型：
--
作者：
E. Senba;M. Tohyama;Sadao Shiosaka;H. Takagi;M. Sakanaka;T. Matsuzaki;Yasuyuki Takahashi;N. Shimizu
本文对大鼠三叉神经脊束核（VSP）内去甲肾上腺素（NA）终末的起源和精细结构进行了研究。结果表明，VSP内NA由双侧蓝斑（LC）和延髓NA神经元群两个NA神经元系统提供。（1）两种荧光纤维（细和粗）在VSP中被识别;（2）Vsp内注射HRP可标记双侧LC和延髓NA区神经元，采用改良的高锰酸钾固定法研究了Vsp内NA终末的精细结构。该区域的NA末端含有大量的小核心囊泡和少量的大核心囊泡。NA终端在这方面发现的最常见的功能是轴-树突状接触。一个较小的，但仍然大量的，与轴突-轴突接触也被注意到，在这方面，应该强调的是，在本研究中检查的非NA终端总是突触前的结构，因为在非NA终端中发现的透明囊泡有时聚集接近接触膜，其中一些融合或接触膜。由于与NA终末关系密切的树突相对较大，且含有大量细胞器，因此推测这些树突为近端节段。在接触区，虽然没有发现典型的突触特化，但显示了以下提示突触样接触的轮廓：（1）接触膜之间有些致密的物质（2）接触后膜附近致密物质的轻微积聚：（3）接触膜的排列紊乱，膜间间隙扩大;和（4）突触囊泡在接触膜处聚集，其中一些融合或接触接触膜。
An attempt was made to elucidate the origins and fine structure of the noradrenaline (NA) terminals in the nucleus tractus spinalis nervi trigemini (Vsp) of the rat.The present study revealed that the NA in the Vsp is supplied from two NA neuron systems; bilateral locus coeruleus (LC) and medullary NA neuron group, on the grounds that: (1) two types of fluorescent fibers (fine and coarse) were identified in the Vsp; and (2) HRP injected into the Vsp resulted in labeling of neurons of the bilateral LC and bulbal NA areas, particularly the ipsilateral A1area.The fine structure of the NA terminals in the Vsp was investigated by a modified potassium permanganate fixation method. The NA terminals in this area contained a number of small cored vesicles together with a small number of large cored vesicles. The most frequent feature of the NA terminals found in this area was axo-dendritic contact. A lesser, but still substantial, number with axo-axonic contact were also noticed, in connection with which, it should be stressed that the non-NA terminals examined in this study were always presynaptic in structure, since the clear vesicles found in the non-NA terminals sometimes aggregated close to the contact membrane, where some of them fused or touched the membrane. As the dendrites showing a close relationship with the NA terminals were relatively large in size and contained a number of cytoplasmic organelles, these dendrites were assumed to be the proximal segments. At the contact zone, although no typical synaptic specialization was identified, the following profiles suggestive of synaptic-like contact were demonstrated: (1) somewhat dense material between contact membranes (intersynaptic filaments); (2) a slight accumulation of the dense materials adjacent to the postcontact membrane; (3) disarrangement of the contact membranes and enlargement of the space between these membranes; and (4) aggregation of the synaptic vesicles at the contact membrane with some of them fusing or touching the contact membrane.