Ratiometric luminescence 2D in vivo imaging and monitoring of mouse skin oxygenation
Ratiometric luminescence 2D in vivo imaging and monitoring of mouse skin oxygenation
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DOI：
10.1088/2050-6120/1/4/045002
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发表时间：
2013-12-01
影响因子：
3.2
通讯作者：
Jantsch, Jonathan
中科院分区：
文献类型：
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作者：
Hofmann, Julian;Meier, Robert J.;Jantsch, Jonathan
组织氧合在各种疾病的发病机制中起着至关重要的作用，但仍需要建立用于其体内测量的非侵入性、稳健且用户友好的方法。在这里，我们提出了一种体内氧检测系统，使用比率发光成像（RLI）作为读出方案，以确定皮肤的小鼠后足垫的氧张力，通过并排比较与更成熟的技术，包括发光寿命成像，使用平面传感器膜和极谱电极作为金标准。我们还证明，这种技术允许检测小鼠皮肤组织氧合的变化引起的小鼠全身缺氧。这些数据表明，基于RLI的氧成像是一种最有用的工具，用于可靠和容易地分析和监测体内皮肤组织的氧合。这项技术将促进我们对各种疾病条件下皮肤组织氧合的局部调节的理解。
Tissue oxygenation plays a critical role in the pathogenesis of various diseases, but non-invasive, robust and user-friendly methods for its measurement in vivo still need to be established. Here, we are presenting an in vivo oxygen-detection system that uses ratiometric luminescence imaging (RLI) as a readout scheme to determine the skin oxygen tension of mouse hind footpads via side-by-side comparison with more established techniques including luminescence-lifetime imaging using planar sensor films and the polarographic electrode as the gold standard. We also demonstrate that this technology allows the detection of changes in mouse skin tissue oxygenation induced by subjecting mice to systemic hypoxia. The data demonstrate oxygen imaging based on RLI to be a most useful tool for reliably and easily analyzing and monitoring skin tissue oxygenation in vivo. This technology will advance our understanding of local regulation of skin tissue oxygenation in various disease conditions.