Underivatized cyclic olefin copolymer as substrate material and stationary phase for capillary and microchip electrochromatography
Underivatized cyclic olefin copolymer as substrate material and stationary phase for capillary and microchip electrochromatography
复制标题
DOI：
10.1002/elps.200800131
复制
发表时间：
2008-08-01
期刊：
影响因子：
2.9
通讯作者：
Kutter, Jorg P.
中科院分区：
文献类型：
--
作者：
Gustafsson, Omar;Mogensen, Klaus B.;Kutter, Jorg P.
我们首次报告使用未衍生化环烯烃共聚物（COC，更具体地说：Topas）作为毛细管和微芯片电色谱（CEC）的基质材料和固定相，并演示了无需涂层程序的色谱分离。 25 Pm id Topas 毛细管中的电渗迁移率测量显示出显着的与 pH 相关的阴极 EOF。电渗迁移率的大小与玻璃基板和其他聚合物材料中的电渗迁移率相当。采用开管 CEC 在板高度范围为 5.3 至 12.7 μm 的未衍生化 Topas 毛细管中对三种中性化合物进行基线分离。使用 254 nm 处的紫外吸光度检测分析物，从而利用 Topas 在短波长下的光学透明度。还介绍了通过纳米压印光刻技术制造基于 Topas 的电色谱微芯片。微芯片的分离柱中有一系列柱子，以增加表面积。成功压印的最小特征约为2μm宽、5μm高。粘合过程中未使用等离子处理，从而保持了原生材料的表面特性。在板高度范围为 3.4 至 22 μm 的未衍生化微芯片上演示了三种荧光标记胺的 RP 微芯片电色谱分离。
We report, for the first time, the use of underivatized cyclic olefin copolymer (COC, more specifically: Topas) as the substrate material and the stationary phase for capillary and microchip electrochromatography (CEC), and demonstrate chromatographic separations without the need of coating procedures. Electroosmotic mobility measurements in a 25 Pm id Topas capillary showed a significant cathodic EOF that is pH-dependent. The magnitude of the electroosmotic mobility is comparable to that found in glass substrates and other polymeric materials. Open-tubular CEC was employed to baseline-separate three neutral compounds in an underivatized Topas capillary with plate heights ranging from 5.3 to 12.7 mu m. The analytes were detected using UV absorbance at 254 nm, thus taking advantage of the optical transparency of Topas at short wavelengths. The fabrication of a Topas-based electrochromatography microchip by nanoimprint lithography is also presented. The microchip has an array of pillars in the separation column to increase the surface area. The smallest features that were successfully imprinted were around 2 mu m wide and 5 Pm high. No plasma treatment was used during the bonding, thus keeping the surface properties of the native material. An RP microchip electrochromatography separation of three fluorescently labeled amines is demonstrated on the underivatized microchip with plate heights ranging from 3.4 to 22 mu m.