A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.
A cytoplasmic factor promoting oocyte maturation: its extraction and preliminary characterization.
复制标题
促进卵母细胞成熟的细胞质因子：其提取和初步表征。
DOI：
10.1126/science.1083070
复制
发表时间：
1976
期刊：
影响因子：
56.9
通讯作者：
Y. Masui
中科院分区：
文献类型：
--
作者：
W. Wasserman;Y. Masui
成熟的两栖类卵母细胞的细胞质中含有一种诱导生殖囊泡破裂的因子。该因子从尖音蛙卵中提取，并通过显微注射非洲爪蟾卵母细胞进行测定。该因子的活性是Mg依赖性的，Ca敏感性的，并且与热不稳定蛋白相关。蔗糖密度梯度电泳显示，该因子存在于三种不同的分子大小。
Cytoplasm of maturing amphibian oocytes possesses a factor that induces germinal vesicle breakdown. This factor was extracted from Rana pipiens eggs and assayed by microinjection in Xenopus laevis oocytes. The activity of this factor is Mg-dependent, Ca-sensitive, and associated with heat-labile protein. Centrifugation on a sucrose density gradient revealed that the factor exists in three different molecular sizes.