Target-cell recognition by cloned lines of influenza virus-specific cytotoxic T lymphocytes. Selective inhibition by a monoclonal H-2-specific antibody.
Target-cell recognition by cloned lines of influenza virus-specific cytotoxic T lymphocytes. Selective inhibition by a monoclonal H-2-specific antibody.
复制标题
流感病毒特异性细胞毒性 T 淋巴细胞克隆系的靶细胞识别。
DOI：
10.1007/bf00375501
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发表时间：
1982
期刊：
影响因子：
3.2
通讯作者：
Hauptfeld,V
中科院分区：
文献类型：
--
作者：
Braciale,TJ;Mojcik,CF;Hauptfeld,V
单克隆H-2d特异性抗体显著抑制由两种流感病毒A/JAP/57特异性、H-2Kd限制性克隆CTL系介导的靶细胞裂解。另外三个A/JAP/57特异性H-2d限制性CTL克隆（其中两个也限制于H-2Kdin靶细胞识别）仅被该单克隆抗体最低限度地抑制。抗体的抑制作用不是由于选择性结合某些克隆的CTL系，而是由于阻断靶细胞上的决定簇。该单克隆抗体产生对由A/JAP/57特异性、H-2d限制性CTL的异质群体介导的裂解的部分抑制。同样，H-2d特异性单克隆抗体产生的细胞溶解的深刻的选择性抑制不能用常规的H-2d同种抗血清再现。这些观察结果表明，一个特定的H-2K或H-2D分子上的多个位点可以作为H-2限制性CTL识别的决定因素。它们进一步暗示，在针对特定抗原的H-2限制性CTL群体中，存在不止一种自身MHC产物的识别结构（受体）。
A monoclonal H-2d-specific antibody markedly inhibits target-cell lysis mediated by two influenza virus A/JAP/57-specific,H-2Kd-restricted cloned CTL lines. Three other A/JAP/57-specific,H-2d-restricted CTL clones (two of which are also restricted toH-2Kdin target-cell recognition) are only minimally inhibited by this monoclonal antibody. The inhibitory effect of the antibody is not due to selective binding to certain cloned CTL lines but rather is due to blocking of a determinant on the target cell. The monoclonal antibody produces partial inhibition of lysis mediated by a heterogeneous population of A/JAP/57-specific,H-2d-restricted CTL. Likewise the profound, selective inhibition of cytolysis produced by the H-2d-specific monoclonal antibody could not be reproduced with a conventional H-2dalloantiserum. These observations suggest that more than one site on a particular H-2K or H-2D molecule can serve as a determinant forH-2-restricted CTL recognition. They furthermore imply that there is more than one recognition structure (receptor) for self MHC products clonally distributed among a population ofH-2-restricted CTL directed to a particular antigen.