Effects of Tricyclazole on Cadmium Tolerance and Accumulation Characteristics of a Dark Septate Endophyte (DSE), Exophiala pisciphila
Effects of Tricyclazole on Cadmium Tolerance and Accumulation Characteristics of a Dark Septate Endophyte (DSE), Exophiala pisciphila
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DOI：
10.1007/s00128-015-1676-4
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发表时间：
2016-02
影响因子：
2.7
通讯作者：
F. Zhan;Yong-mei He;Yi-Ying Yang;Yuan Li;Tao Li;Zhiwei Zhao
中科院分区：
文献类型：
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作者：
F. Zhan;Yong-mei He;Yi-Ying Yang;Yuan Li;Tao Li;Zhiwei Zhao
嗜鱼外瓶霉（Exophialapisciphilais）是一种耐镉真菌，能产生1，8-二羟基萘黑色素，三环唑能抑制该黑色素的产生。较高浓度（20和40 µg mL−1）的三环唑可降低大肠杆菌的生长和孢子形成。但在低浓度（2.5 µg mL−1）下未观察到毒性。在镉（Cd）胁迫（50、100和200 mg L−1）下，2.5 µg mL− 1的三环唑可减少真菌生长和孢子形成。这些降低表明E.鱼友对于0和2.5 µg mL− 1三环唑处理，Cd主要与细胞壁结合，并被2%乙酸和1 M NaCl萃取。红外光谱分析表明，E. 0和2.5 µg mL− 1三环唑处理的亲鱼性菌丝相似，显示羟基、胺、羧基和磷酸基团。因此，三环唑抑制黑色素合成并不改变E.鱼友结果表明，黑色素对E.但黑色素合成的抑制对Cd在E.鱼友
Exophiala pisciphilais a cadmium-tolerant fungus, and produces 1,8-dihydroxynaphthalene melanin which can be inhibited by tricyclazole. Tricyclazole at higher levels (20 and 40 µg mL−1) reduced the growth and sporulation ofE. pisciphila, but toxicity was not observed at a low concentration (2.5 µg mL−1). Under cadmium (Cd) stress (50, 100 and 200 mg L−1), 2.5 µg mL−1of tricyclazole reduced fungal growth and sporulation. These reduces indicated a decrease on Cd tolerance ofE. pisciphila. For both the 0 and 2.5 µg mL−1tricyclazole treatments, Cd was associated mostly with cell walls and was extracted by 2 % acetic acid and 1 M NaCl. The FTIR spectra of theE. pisciphilamycelia were similar for both 0 and 2.5 µg mL−1tricyclazole treatments, which showed hydroxyl, amine, carboxyl and phosphate groups. Thus inhibition of melanin synthesis by tricyclazole did not change Cd accumulation characteristics inE. pisciphila. Results suggested that melanin played a protective role forE. pisciphilaagainst Cd stress, but inhibition of melanin synthesis did not have a remarkable impact on Cd accumulation inE. pisciphila.