In vitro expansion of a multipotent population of human neural progenitor cells
In vitro expansion of a multipotent population of human neural progenitor cells
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DOI：
10.1006/exnr.1999.7098
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发表时间：
1999-08-01
影响因子：
5.3
通讯作者：
Wahlberg, LU
中科院分区：
文献类型：
--
作者：
Carpenter, MK;Cui, X;Wahlberg, LU
人神经祖细胞的分离和扩增具有重要的潜在临床应用，因为这些细胞可用作细胞疗法中的移植材料，以在患有中枢神经系统（CNS）病症的患者中再生组织和/或功能。本文描述了人胚胎前脑中连续分裂的多能祖细胞群，可以在体外繁殖。这些细胞可以使用含有碱性成纤维细胞生长因子（bFGF）、白血病抑制因子（LIF）和表皮生长因子（EGF）的无血清限定培养基来维持和扩增。使用这三种因子，细胞培养物扩增并在体外保持多能性至少1年。该扩增期导致该异质细胞群增加10（7）倍。在分化时，它们形成神经元、星形胶质细胞和少突胶质细胞，这是CNS中的三种主要表型。此外，GABA免疫反应和酪氨酸羟化酶免疫反应的神经元可以确定。这些结果证明了长期体外扩增人神经祖细胞的可行性。用于同种异体移植的这种神经前体群体的优点包括提供可扩展的、充分表征的、确定的细胞来源的能力，所述细胞来源可以形成特定的神经元或神经胶质亚型。(C)北京：科学出版社.
The isolation and expansion of human neural progenitor cells have important potential clinical applications, because these cells may be used as graft material in cell therapies to regenerate tissue and/or function in patients with central nervous system (CNS) disorders. This paper describes a continuously dividing multipotent population of progenitor cells in the human embryonic forebrain that can be propagated in vitro. These cells can be maintained and expanded using a serum-free defined medium containing basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), and epidermal growth factor (EGF). Using these three factors, the cell cultures expand and remain multipotent for at least 1 year in vitro. This period of expansion results in a 10(7)-fold increase of this heterogeneous population of cells. Upon differentiation, they form neurons, astrocytes, and oligodendrocytes, the three main phenotypes in the CNS. Moreover, GABA-immunoreactive and tyrosine hydroxylase-immunoreactive neurons can be identified. These results demonstrate the feasibility of long-term in vitro expansion of human neural progenitor cells. The advantages of such a population of neural precursors for allogeneic transplantation include the ability to provide an expandable, well-characterized, defined cell source which can form specific neuronal or glial subtypes. (C) 1999 Academic Press.