A pilot study to compare the detection of HPV-16 biomarkers in salivary oral rinses with tumour p16INK4a expression in head and neck squamous cell carcinoma patients
A pilot study to compare the detection of HPV-16 biomarkers in salivary oral rinses with tumour p16INK4a expression in head and neck squamous cell carcinoma patients
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DOI：
10.1186/s12885-016-2217-1
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发表时间：
2016-03-03
期刊：
影响因子：
3.8
通讯作者：
Punyadeera, Chamindie
中科院分区：
文献类型：
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作者：
Chai, Ryan C.;Lim, Yenkai;Punyadeera, Chamindie
背景：人乳头瘤病毒 16 (HPV-16) 感染是头颈鳞状细胞癌 (HNSCC)，特别是口咽鳞状细胞癌 (OPSCC) 的主要危险因素。目前评估 HNSCC 中 HPV-16 状态的技术包括检测肿瘤组织中的 HPV-16 DNA 和 p16(INK4a) 表达。当肿瘤起源于隐藏的解剖部位时，这种方法可能具有挑战性。因此，对于 HPV-16 检测，需要一种非侵入性且经济高效的活检替代方案，尤其是在社区环境中筛查高危个体。方法：本研究使用终点和定量聚合酶链反应 (PCR) 对 82 名 HNSCC 患者的唾液口腔冲洗液中 HPV-16 DNA 和 RNA 的检测与肿瘤 p16(INK4a) 状态进行了比较。结果：在 42 名 p16(INK4a) 阳性患者中使用终点和定量 PCR，39 例（敏感性 = 92.9%、PPV = 100% 和 NPV = 93%）的口腔冲洗样本中可检测到 HPV-16 DNA。在 40 名 p16(INK4a) 阴性肿瘤患者的口腔冲洗液样本中未检测到 HPV-16 DNA，测试特异性为 100%。对于 p16(INK4a) 阳性肿瘤患者，使用终点逆转录 PCR (RT-PCR) 检测到 HPV-16 mRNA 的比例为 24/40（灵敏度 = 60 %，PPV = 100 % 和 NPV = 71 %），使用定量 RT-PCR 检测到的比例为 22/40（灵敏度 = 55 %，PPV = 100 % 和 NPV = 69） %）。在 p16(INK4a) 阴性患者的口腔冲洗液样本中未检测到 HPV-16 mRNA，特异性为 100%。结论：我们证明，唾液口腔冲洗液中 HPV-16 DNA 的检测可以指示 HNSCC 患者的 HPV 状态，并且除当前方法外，还可以用作诊断工具。
Background: Human papilloma virus-16 (HPV-16) infection is a major risk factor for a subset of head and neck squamous cell carcinoma (HNSCC), in particular oropharyngeal squamous cell carcinoma (OPSCC). Current techniques for assessing the HPV-16 status in HNSCC include the detection of HPV-16 DNA and p16(INK4a) expression in tumor tissues. When tumors originate from hidden anatomical sites, this method can be challenging. A non-invasive and cost-effective alternative to biopsy is therefore desirable for HPV-16 detection especially within a community setting to screen at-risk individuals.Methods: The present study compared detection of HPV-16 DNA and RNA in salivary oral rinses with tumor p16(INK4a) status, in 82 HNSCC patients using end-point and quantitative polymerase chain reaction (PCR).Results: Of 42 patients with p16(INK4a)-positive tumours, 39 (sensitivity = 92.9 %, PPV = 100 % and NPV = 93 %) had oral rinse samples with detectable HPV-16 DNA, using end-point and quantitative PCR. No HPV-16 DNA was detected in oral rinse samples from 40 patients with p16(INK4a) negative tumours, yielding a test specificity of 100 %. For patients with p16(INK4a) positive tumours, HPV-16 mRNA was detected using end-point reverse transcription PCR (RT-PCR) in 24/40 (sensitivity = 60 %, PPV = 100 % and NPV = 71 %), and using quantitative RT-PCR in 22/40 (sensitivity = 55 %, PPV = 100 % and NPV = 69 %). No HPV-16 mRNA was detected in oral rinse samples from the p16(INK4a) -negative patients, yielding a specificity of 100 %.Conclusions: We demonstrate that the detection of HPV-16 DNA in salivary oral rinse is indicative of HPV status in HNSCC patients and can potentially be used as a diagnostic tool in addition to the current methods.