Endothelial nitric oxide synthase in placental villous tissue from normal, pre-eclamptic and intrauterine growth restricted pregnancies
Endothelial nitric oxide synthase in placental villous tissue from normal, pre-eclamptic and intrauterine growth restricted pregnancies
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DOI：
10.1093/humrep/12.1.167
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发表时间：
1997-01-01
影响因子：
6.1
通讯作者：
Lyall, F
中科院分区：
文献类型：
--
作者：
Myatt, L;Eis, ALW;Lyall, F
一氧化氮（NO）在人类胎盘中如何调节血液。在妊娠合并先兆子痫和/或宫内生长受限（IUGR）的胎儿-胎盘血管系统中观察到血流阻力增加，我们检测了这些胎盘中内皮型一氧化氮合酶（eNOS）的表达。从正常血压对照（n = 5）、单独IUGR（n = 5）或先兆子痫（伴或不伴IUGR）（n = 9）患者获得胎盘绒毛组织切片，对eNOS进行免疫染色，并对合体滋养层和胎盘血管中eNOS染色的定位、类型（点状或弥漫性）和强度进行评分。使用Mann-Whitney U检验计算差异的显著性。在合体滋养层中的免疫染色强度或类型没有差异，先兆子痫伴或不伴IUGR患者的胎盘中eNOS在合体滋养层中的分布明显更基底。eNOS免疫组化染色在正常胎盘的绒毛毛细血管末端和绒毛血管内皮中均不存在，而在正常胎盘的绒毛血管内皮中eNOS免疫组化染色较弱，但在IUGR和先兆子痫组的这两种类型的血管内皮中eNOS免疫组化染色较强，在单独IUGR患者的绒毛血管内皮中eNOS免疫组化染色明显更强。这种增加的eNOS表达和因此增加的NO产生在胎儿胎盘血管系统中可能是对这些病理性妊娠中增加的阻力和不良灌注的适应性反应。
Nitric oxide (NO) regulates blood how in the human placenta. Gs increased resistance to blood flow is seen in the fetal-placental vasculature in pregnancies complicated by pre-eclampsia and/or intrauterine growth restriction (IUGR), we examined expression of endothelial nitric oxide synthase (eNOS) in these placentas. Placental villous tissue sections were obtained from normotensive control (n = 5), IUGR alone (n = 5) or pre eclamptic (with or without IUGR) (n = 9) patients, immunostained for eNOS and scored for localization, type (punctate or diffuse) and intensity of eNOS staining in syncytiotrophoblast and placental vessels. The significance of differences was calculated using the Mann-Whitney U-test. No differences in intensity or type of immunostaining in syncytiotrophoblast were seen, placentas from patients with pre-eclampsia with or without IUGR had a significantly more basal distribution of eNOS in syncytiotrophoblast. eNOS immunostaining was absent in terminal villous capillary and faint in stem villous vessel endothelium of normal placentas, but was intense in the endothelium of both of these types of vessels in the IUGR and pre-eclampsia groups, with significantly greater staining seen in stem vessels of patients with IUGR alone. This increased eNOS expression and hence increased NO production in the fetal-placental vasculature may be an adaptive response to the increased resistance and poor perfusion in these pathological pregnancies.