Prasinoviruses of the Marine Green Alga Ostreococcus tauri Are Mainly Species Specific
Prasinoviruses of the Marine Green Alga Ostreococcus tauri Are Mainly Species Specific
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DOI：
10.1128/jvi.07221-11
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发表时间：
2012-04-01
影响因子：
5.4
通讯作者：
Grimsley, Nigel
中科院分区：
文献类型：
--
作者：
Clerissi, Camille;Desdevises, Yves;Grimsley, Nigel
感染单胞绿藻目（单胞绿藻纲）的prasinovirus常见于其寄主种类较多的沿海海水中。我们在13个独立分离的野生型牛链球菌、4个野生型lucimarinus菌株、1个地中海Ostreococcus sp.（“Ostreococcus mediterraneus”）分支D菌株以及Mamiellales、Bathycoccus prasinos和Micromonas pusilla中另外两个亲缘种各1个代表种上检测了40种牛链球菌病毒。40种病毒中有34种只感染牛牛球绦虫，5种能感染其他一种Ostreococcus属，1种能感染另外两种Ostreococcus属，但均未感染其他属。我们观察到，Ostreococcus菌株对病毒的总体易感性模式与已知显示种内大小变化的两条宿主染色体的大小有关，遗传相关的病毒倾向于感染相同的宿主菌株，并且携带毒素的病毒严格具有菌株特异性。比较两个完整的牛头牛病毒蛋白质组揭示了至少三个预测蛋白是候选病毒特异性决定因素。
Prasinoviruses infecting unicellular green algae in the order Mamiellales (class Mamiellophyceae) are commonly found in coastal marine waters where their host species frequently abound. We tested 40 Ostreococcus tauri viruses on 13 independently isolated wild-type O. tauri strains, 4 wild-type O. lucimarinus strains, 1 Ostreococcus sp. ("Ostreococcus mediterraneus") clade D strain, and 1 representative species of each of two other related species of Mamiellales, Bathycoccus prasinos and Micromonas pusilla. Thirty-four out of 40 viruses infected only O. tauri, 5 could infect one other species of the Ostreococcus genus, and 1 infected two other Ostreococcus spp., but none of them infected the other genera. We observed that the overall susceptibility pattern of Ostreococcus strains to viruses was related to the size of two host chromosomes known to show intraspecific size variations, that genetically related viruses tended to infect the same host strains, and that viruses carrying inteins were strictly strain specific. Comparison of two complete O. tauri virus proteomes revealed at least three predicted proteins to be candidate viral specificity determinants.