CELLULOSE FERMENTATION BY A RUMEN ANAEROBIC FUNGUS IN BOTH THE ABSENCE AND THE PRESENCE OF RUMEN METHANOGENS
CELLULOSE FERMENTATION BY A RUMEN ANAEROBIC FUNGUS IN BOTH THE ABSENCE AND THE PRESENCE OF RUMEN METHANOGENS
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DOI：
10.1128/aem.42.6.1103-1110.1981
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发表时间：
1981-01-01
影响因子：
4.4
通讯作者：
MOUNTFORT, DO
中科院分区：
文献类型：
--
作者：
BAUCHOP, T;MOUNTFORT, DO
本文描述了在瘤胃产甲烷菌存在和不存在的情况下，绵羊瘤胃厌氧真菌对纤维素的发酵。在单一培养物中，作为发酵的己糖的摩尔百分比的产物的摩尔数是：乙酸，72.7; CO2 37.6;甲酸，83.1;乙醇，37.4;乳酸，67.0;和H2，35.3。在共培养中，乙酸是主要产物（134.7%），CO2增加（88.7%）。乳酸和乙醇产量分别下降至2.9%和19%，检测到少量甲酸（1%），H2没有积累。在共培养物中产生大量的甲烷（58.7%）。用[2- 14 C]乙酸盐进行的研究表明，乙酸盐不是甲烷的前体。真菌纤维素发酵的证明扩展了能够参与纤维素消化的已知瘤胃生物的范围，并为厌氧真菌在瘤胃纤维消化中的作用提供了进一步的支持。产甲烷菌对发酵模式的影响被解释为电子流从电子汇产物经由H2向甲烷的转移。讨论了一个新的微生物间H2转移的例子和第一次由真菌产生H2的证明。
The fermentation of cellulose by an ovine rumen anaerobic fungus in the absence and presence of rumen methanogens is described. In the monoculture, mol of product as a percentage of the mol of hexose fermented were: acetate, 72.7; CO2 37.6; formate, 83.1; ethanol, 37.4; lactate, 67.0; and H2, 35.3. In the coculture, acetate was the major product (134.7%); CO2 increased (88.7%). Lactate and ethanol production decreased to 2.9 and 19%, respectively, little formate was detected (1%) and H2 did not accumulate. Substantial amounts of methane were produced in the coculture (58.7%). Studies with [2-14C]acetate indicated that acetate was not a precursor of methane. The demonstration of cellulose fermentation by a fungus extends the range of known rumen organisms capable of participating in cellulose digestion and provides further support for a role of anaerobic fungi in rumen fiber digestion. The effect of the methanogens on the pattern of fermentation is interpreted as a shift in flow of electrons away from electron sink products to methane via H2. A new example of intermicrobial H2 transfer and the 1st demonstration of H2 formation by a fungus were discussed.