Optical traps to study properties of molecular motors.
Optical traps to study properties of molecular motors.
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用于研究分子马达特性的光陷阱。
DOI：
10.1101/pdb.top066662
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发表时间：
2011
影响因子：
--
通讯作者：
Warrick,HansM
中科院分区：
文献类型：
--
作者：
Spudich,JamesA;Rice,SarahE;Rock,RonaldS;Purcell,ThomasJ;Warrick,HansM
在体外，运动性分析能够分析ATP水解和肌球蛋白沿肌动蛋白细丝或肌动蛋白沿微管移动之间的耦合。使用激光捕获的单分子分析已经被用来获得关于运动蛋白、肌球蛋白和过程DNA酶的更详细的信息。结合体外运动性分析和激光陷阱测量，揭示了与ATPase循环相关的详细动态结构变化。本文介绍了利用光学陷阱研究过程中和非过程中的分子马达蛋白，重点介绍了仪器的设计和表征运动性的方法。
In vitro motility assays enabled the analysis of coupling between ATP hydrolysis and movement of myosin along actin filaments or kinesin along microtubules. Single-molecule assays using laser trapping have been used to obtain more detailed information about kinesins, myosins, and processive DNA enzymes. The combination of in vitro motility assays with laser-trap measurements has revealed detailed dynamic structural changes associated with the ATPase cycle. This article describes the use of optical traps to study processive and nonprocessive molecular motor proteins, focusing on the design of the instrument and the assays to characterize motility.