Survivin subcellular localization in high-grade astrocytomas: simultaneous expression in both nucleus and cytoplasm is negative prognostic marker
Survivin subcellular localization in high-grade astrocytomas: simultaneous expression in both nucleus and cytoplasm is negative prognostic marker
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DOI：
10.1007/s11060-006-9267-1
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发表时间：
2007-04-01
影响因子：
3.9
通讯作者：
Kurisu, Kaoru
中科院分区：
文献类型：
--
作者：
Saito, Taiichi;Arifin, Muhamad Thohar;Kurisu, Kaoru
目的探讨生存素（survivin）在恶性肿瘤中的作用及其与预后的关系。然而，目前还没有关于高级别星形细胞瘤中细胞下定位生存素的意义的报道。本研究的目的是研究预后和生存素在高级别astrocytoma.Methods的亚细胞定位的关系，我们化学检查的生存素表达（核，胞浆，或两者兼而有之）在51例高级别星形细胞瘤（19间变性星形细胞瘤，32胶质母细胞瘤）的亚细胞定位的模式。我们使用包括其他临床病理因素的多变量分析，统计学上研究了生存素定位和预后之间的关系结果所有标本均为Survivin阳性表达，仅定位于细胞核，表达强度与肿瘤大小、肿瘤大小、组织学分级、MIB-1标记指数、p53和表皮生长因子受体（epidermal growth factor receptor，EGFR）的表达密切相关结果：10例（20%）为胞浆阳性，23例（45%）为胞浆阳性，19例（35%）为胞核和胞浆同时表达。核阳性组与胞浆阳性组的预后差异无统计学意义（P = 0.796）。而胞浆核阳性组的生存期明显短于胞浆阳性组和胞核阳性组（P < 0.0001）。结论Survivin在高级别星形细胞瘤中的表达是一个重要的预后因素。目前的研究结果表明，生存素表达的亚细胞定位是一个可靠的预后因素与这种肿瘤的患者。
Objective Subcellularly localized (nuclear and/or cytoplasmic) survivin has various functions, and correlates with prognosis of malignant tumors. However, there have been no reports about the significance of subcellularly localized survivin in high-grade astrocytomas. The aim of the present study was to examine the relationship between prognosis and subcellular localization of survivin in high-grade astrocytoma.Methods We immunohistochemically examined the pattern of subcellular localization of survivin expression (nuclear, cytoplasmic, or both) in 51 patients with high-grade astrocytoma (19 anaplastic astrocytomas; 32 glioblastomas). We statistically examined the relationship between survivin localization and prognosis, using multivariate analysis including other clinicopathological factors (age, sex, WHO grade, extent of resection, MIB-1 labeling index, and expression of p53 and epidermal growth factor receptor).Results All specimens stained positive for survivin: localized in nucleus only (nuclear-positive group), 10 cases (20%); localized in cytoplasm only (cytoplasmic-positive group), 23 cases (45%); simultaneous expression in nucleus and cytoplasm (nuclear-cytoplasmic group), 19 cases (35%). There was no significant difference in prognosis between the nuclear-positive group and cytoplasmic-positive group (P = 0.796). However, the nuclear-cytoplasmic group had significantly shorter overall survival than the nuclear-positive group and the cytoplasmic-positive group (P < 0.0001).Conclusions We found that simultaneous expression of survivin in both the nucleus and cytoplasm is an important prognostic factor for high-grade astrocytoma. The present findings indicate that subcellular localization of survivin expression is a reliable prognostic factor for patients with this tumor.