Securing the Exchange of Synthetic Genetic Constructs Using Digital Signatures
Securing the Exchange of Synthetic Genetic Constructs Using Digital Signatures
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DOI：
10.1021/acssynbio.0c00401
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发表时间：
2020-10-16
影响因子：
4.7
通讯作者：
Peccoud, Jean
中科院分区：
文献类型：
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作者：
Gallegos, Jenna E.;Kar, Diptendu M.;Peccoud, Jean
合成生物学领域依赖于不断增长的合成遗传物质供应链。确保这种材料的交换的技术仍处于初级阶段。到目前为止，提出的解决方案主要集中在水印上，这是一种过时的安全方法，可以用来声称作者身份，但容易被假冒，并且没有提供任何关于遗传物质本身完整性的信息。在这份手稿中，我们描述了如何使用数据加密和数字签名算法来确保合成基因结构的完整性和真实性。使用一个为质粒生成数字签名和其他加密数据的试点软件，我们证明了我们可以预测地从没有参考序列的测序数据中提取关于作者、身份、质粒序列的完整性甚至注释的信息，所有这些都不会损害质粒的功能。将数字签名编码到DNA分子中，为基因设计师提供了一种声称DNA分子的作者的途径。这项技术有助于遵守材料转让协议和其他许可协议。
The field of synthetic biology relies on an ever-growing supply chain of synthetic genetic material. Technologies to secure the exchange of this material are still in their infancy. Solutions proposed thus far have focused on watermarks, a dated security approach that can be used to claim authorship, but is subject to counterfeit, and does not provide any information about the integrity of the genetic material itself. In this manuscript, we describe how data encryption and digital signature algorithms can be used to ensure the integrity and authenticity of synthetic genetic constructs. Using a pilot software that generates digital signatures and other encrypted data for plasmids, we demonstrate that we can predictably extract information about the author, the identity, the integrity of plasmid sequences, and even annotations from sequencing data alone without a reference sequence, all without compromising the function of the plasmids. Encoding a digital signature into a DNA molecule provides an avenue for genetic designers to claim authorship of DNA molecules. This technology could help compliance with material transfer agreements and other licensing agreements.