Obestatin inhibits motor activity in the antrum and duodenum in the fed state of conscious rats
Obestatin inhibits motor activity in the antrum and duodenum in the fed state of conscious rats
复制标题
DOI：
10.1152/ajpgi.00549.2007
复制
发表时间：
2008-05-01
影响因子：
4.5
通讯作者：
Fujimiya, Mineko
中科院分区：
文献类型：
--
作者：
Ataka, Koji;Inui, Akio;Fujimiya, Mineko
Obestatin抑制清醒大鼠进食状态下胃窦和十二指肠的运动活动。美国胃肠和肝脏生理学杂志294：G1210-G1218，2008。首次发表于2008年3月6日;doi：10.1152/ajpgi.00549.2007。Obestatin是由Ghrelin前体基因编码的一种新的多肽，但其对胃肠动力的影响仍存在争议。在这里，我们研究了肥胖抑制素对自由活动的清醒大鼠的胃和十二指肠的进食和禁食运动活动的影响。我们研究了静脉注射（IV）obestatin对胃窦和十二指肠运动指数百分比（% MI）和III期样收缩的影响。同时探讨了obestatin影响胃十二指肠运动的脑机制及迷走神经传入通路的参与。静脉注射后30和90分钟之间，obestatin降低% MI的胃窦和延长的时间恢复到禁食运动在十二指肠的喂养大鼠给予3 g的食物后禁食18小时。免疫组织化学分析表明，促肾上腺皮质激素释放因子和尿皮质素-2在下丘脑室旁核的神经元被激活，通过静脉注射obestatin。脑室内注射CRF 1型和2型受体拮抗剂可阻止obestatin对胃十二指肠运动的影响。辣椒素治疗阻断了obestatin对十二指肠运动的影响，但对胃窦运动没有影响。Obestatin不能拮抗ghrelin诱导的胃十二指肠运动的刺激。这些结果表明，在进食状态下，obestatin抑制胃窦和十二指肠的运动活动，脑中的CRF 1型和2型受体可能参与obestatin对胃十二指肠运动的这些影响。
Obestatin inhibits motor activity in the antrum and duodenum in the fed state of conscious rats. Am J Physiol Gastrointest Liver Physiol 294: G1210-G1218, 2008. First published March 6, 2008;doi:10.1152/ajpgi.00549.2007.-Obestatin is a novel peptide encoded by the ghrelin precursor gene; however, its effects on gastrointestinal motility remain controversial. Here we have examined the effects of obestatin on fed and fasted motor activities in the stomach and duodenum of freely moving conscious rats. We examined the effects of intravenous (IV) injection of obestatin on the percentage motor index (% MI) and phase III- like contractions in the antrum and duodenum. The brain mechanism mediating the action of obestatin on gastroduodenal motility and the involvement of vagal afferent pathway were also examined. Between 30 and 90 min after IV injection, obestatin decreased the % MI in the antrum and prolonged the time taken to return to fasted motility in the duodenum in fed rats given 3 g of chow after 18 h of fasting. Immunohistochemical analysis demonstrated that corticotropin-releasing factor- and urocortin-2-containing neurons in the paraventricular nucleus in the hypothalamus were activated by IV injection of obestatin. Intracerebroventricular injection of CRF type 1 and type 2 receptor antagonists prevented the effects of obestatin on gastroduodenal motility. Capsaicin treatment blocked the effects of obestatin on duodenal motility but not on antral motility. Obestatin failed to antagonize ghrelin-induced stimulation of gastroduodenal motility. These results suggest that, in the fed state, obestatin inhibits motor activity in the antrum and duodenum and that CRF type 1 and type 2 receptors in the brain might be involved in these effects of obestatin on gastroduodenal motility.