FGF-mediated induction of ciliary body tissue in the chick eye
FGF-mediated induction of ciliary body tissue in the chick eye
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DOI：
10.1016/j.ydbio.2006.12.033
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发表时间：
2007-04-01
影响因子：
2.7
通讯作者：
Hyer, Jeanette
中科院分区：
文献类型：
--
作者：
Dias da Silva, Magnus R.;Tiffin, Nicola;Hyer, Jeanette
在形态发生时，视泡的简单神经上皮在脊椎动物视杯中产生四种基本组织：色素上皮、感觉神经视网膜、分泌性睫状体和肌性虹膜。色素上皮和神经视网膜是通过与特定环境和信号的相互作用建立的：眼周间充质/BMP指定色素上皮，表面外胚层/FGF指定神经视网膜。前部（虹膜和睫状体）通过与透镜的相互作用来指定，尽管诱导的分子机制尚未被破译。由于透镜是FGF的来源，我们检查了该因子是否参与诱导睫状体。我们迫使胚胎鸡眼的色素上皮表达FGF 4。受感染的细胞及其近邻细胞转化为神经视网膜。在距离FGF信号一定距离处，组织转变回色素上皮。在移行区发现睫状体组织。异位睫状体从未与透镜组织接触。为了评估透镜对正常睫状体规格的贡献，我们创建了光学杯，其中透镜已被移除，同时仍然是前透镜外胚层。在无晶状体视杯的前部发现睫状体组织。我们建议，睫状体可以指定在视泡阶段，在相同的发育阶段时，神经视网膜和色素上皮细胞被指定，我们提出了一个模型，这可以通过重叠的BMP和FGF信号来实现。(c)2007年爱思唯尔公司All rights reserved.
Upon morphogenesis, the simple neuroepithelium of the optic vesicle gives rise to four basic tissues in the vertebrate optic cup: pigmented epithelium, sensory neural retina, secretory ciliary body and muscular iris. Pigmented epithelium and neural retina are established through interactions with specific environments and signals: periocular mesenchyme/BMP specifies pigmented epithelium and surface ectoderm/FGF specifies neural retina. The anterior portions (iris and ciliary body) are specified through interactions with lens although the molecular mechanisms of induction have not been deciphered. As lens is a source of FGF, we examined whether this factor was involved in inducing ciliary body. We forced the pigmented epithelium of the embryonic chick eye to express FGF4. Infected cells and their immediate neighbors were transformed into neural retina. At a distance from the FGF signal, the tissue transitioned back into pigmented epithelium. Ciliary body tissue was found in the transitioning zone. The ectopic ciliary body was never in contact with the lens tissue. In order to assess the contribution of the lens on the specification of normal ciliary body, we created optic cups in which the lens had been removed while still pre-lens ectoderm. Ciliary body tissue was identified in the anterior portion of lens-less optic cups. We propose that the ciliary body may be specified at optic vesicle stages, at the same developmental stage when the neural retina and pigmented epithelium are specified and we present a model as to how this could be accomplished through overlapping BMP and FGF signals. (c) 2007 Elsevier Inc. All rights reserved.