miR-206 is required for changes in cell adhesion that drive muscle cell morphogenesis in Xenopus laevis.
miR-206 is required for changes in cell adhesion that drive muscle cell morphogenesis in Xenopus laevis.
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DOI：
10.1016/j.ydbio.2018.03.021
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发表时间：
2018-06
影响因子：
2.7
通讯作者：
H. Vergara;Julio Ramirez;Trista Rosing;Ceazar Nave;R. Blandino;D. Saw;Parag Saraf;Gabriel Piexoto;Coohleen Coombes;M. Adams;C. Domingo
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文献类型：
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作者：
H. Vergara;Julio Ramirez;Trista Rosing;Ceazar Nave;R. Blandino;D. Saw;Parag Saraf;Gabriel Piexoto;Coohleen Coombes;M. Adams;C. Domingo
MicroRNAs （miRNAs）是高度保守的非编码小RNA分子，在多细胞生物中转录后调节基因表达。在一组肌肉特异性mirna中，miR-206的表达主要局限于骨骼肌，并且仅在硬骨鱼谱系中发现。尽管许多研究表明miR-206与肌肉维持和疾病有关，但其在骨骼肌发育中的作用在很大程度上仍然未知。在这里，我们研究了miR-206在非洲爪蟾体细胞发生中的作用。在非洲爪蟾中，miR-206的表达与躯体发生的开始一致。我们发现miR-206的敲低和过表达都会导致异常体形成，影响肌肉细胞的旋转、附着和伸长。特别是，我们的数据表明，miR-206调节细胞粘附的变化，影响新形成的体粘附脊索和体间边界的能力。此外，我们发现β-三磷酸糖酐和f -肌动蛋白的表达水平显著降低，这表明miR-206水平的下调影响了细胞形状变化和附着所需的细胞力学，而这些是适当肌肉形成所必需的。
MicroRNAs (miRNAs) are highly conserved small non-coding RNA molecules that post-transcriptionally regulate gene expression in multicellular organisms. Within the set of muscle-specific miRNAs, miR-206 expression is largely restricted to skeletal muscle and is found exclusively within the bony fish lineage. Although many studies have implicated miR-206 in muscle maintenance and disease, its role in skeletal muscle development remains largely unknown. Here, we examine the role of miR-206 duringXenopus laevissomitogenesis. InXenopus laevis,miR-206 expression coincides with the onset of somitogenesis. We show that both knockdown and over-expression of miR-206 result in abnormal somite formation affecting muscle cell rotation, attachment, and elongation. In particular, our data suggests that miR-206 regulates changes in cell adhesion that affect the ability of newly formed somites to adhere to the notochord as well as to the intersomitic boundaries. Additionally, we show that β-dystroglycan and F-actin expression levels are significantly reduced, suggesting that knockdown of miR-206 levels affects cellular mechanics necessary for cell shape changes and attachments that are required for proper muscle formation.