INHIBITION OF LYMPHOCYTE-PROLIFERATION BY A SYNTHETIC PEPTIDE HOMOLOGOUS TO RETROVIRAL ENVELOPE PROTEINS
INHIBITION OF LYMPHOCYTE-PROLIFERATION BY A SYNTHETIC PEPTIDE HOMOLOGOUS TO RETROVIRAL ENVELOPE PROTEINS
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DOI：
10.1126/science.2996136
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
SNYDERMAN, R
中科院分区：
文献类型：
--
作者：
CIANCIOLO, GJ;COPELAND, TD;SNYDERMAN, R
逆转录病毒跨膜包膜蛋白p15 E是免疫抑制性的，因为它抑制淋巴细胞、单核细胞和巨噬细胞的免疫应答。p15 E区域在鼠和猫逆转录病毒中是保守的;在人逆转录病毒HTLV-I和HTLV-II的跨膜包膜蛋白以及由内源性C型人逆转录病毒DNA编码的推定包膜蛋白中也发现了同源区域。合成了与该同源区对应的肽（CKS-17），并检查了其对淋巴细胞增殖的影响。CKS-17抑制白细胞介素-2依赖性鼠细胞毒性T细胞系的增殖以及同种异体抗原刺激的鼠和人淋巴细胞的增殖。其他四种肽，代表病毒蛋白质的不同区域，是无活性的。这些结果表明，逆转录病毒跨膜包膜蛋白的免疫抑制部分可能驻留，至少部分，在一个保守的序列所代表的CKS-17肽。
The retroviral transmembrane envelope protein p15E is immunosuppressive in that it inhibits immune responses of lymphocytes, monocytes, and macrophages. A region of p15E has been conserved among murine and feline retroviruses; a homologous region is also found in the transmembrane envelope proteins of the human retroviruses HTLV-I and HTLV-II and in a putative envelope protein encoded by an endogenous C-type human retroviral DNA. A peptide (CKS-17) was synthesized to correspond to this region of homology and was examined for its effects on lymphocyte proliferation. CKS-17 inhibited the proliferation of an interleukin-2-dependent murine cytotoxic T-cell line as well as alloantigen-stimulated proliferation of murine and human lymphocytes. Four other peptides, representing different regions of virus proteins, were inactive. These results suggest that the immunosuppressive portion of retroviral transmembrane envelope proteins may reside, at least in part, in a conserved sequence represented by the CKS-17 peptide.