Green light induces transcription of the phycoerythrin operon in the cyanobacterium Calothrix 7601.
Green light induces transcription of the phycoerythrin operon in the cyanobacterium Calothrix 7601.
复制标题
绿光诱导蓝藻 Calothrix 7601 中藻红蛋白操纵子的转录。
DOI：
10.1093/nar/14.21.8279
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发表时间：
1986
影响因子：
14.9
通讯作者：
TandeaudeMarsac,N
中科院分区：
文献类型：
--
作者：
Mazel,D;Guglielmi,G;Houmard,J;Sidler,W;Bryant,DA;TandeaudeMarsac,N
藻胆体是蓝藻的主要捕光复合体，是由发色蛋白（藻胆蛋白）和非发色连接多肽组成的多分子结构。本文报道了1nCalothrixPCC 7601藻红蛋白α和β亚基基因cpeA和cpeB的分离和核苷酸序列。在CalothrixPCC 7601中，藻胆素体的多肽组成的调节响应于光波长的变化而发生，这种现象称为互补色适应。在绿色照明下，细胞合成藻红蛋白及其两个特异性相关的接头多肽（L35 R和L36 R），而在红色照明下，这些蛋白质均未检测到。使用特异性探针，检测到对应于cpe基因的单个转录物（1450个核苷酸长），但仅在绿光生长的细胞中，建立了响应于光波长变化的该操纵子表达的转录调节的发生。该转录本的大小排除了藻蓝蛋白相关的L35 R和L36 R可能与thepeA和cpeB基因共转录的可能性。
Phycobilisomes, the major light-harvesting complexes of cyanobacteria are multimolecular structures made up of chromophoric proteins called phycobi1i proteins and non chromophoric linker polypeptides. We report here the isolation and nucleotide sequence of the genes,cpeAandcpeB, which 1nCalothrixPCC 7601 encode the α and β subunits of phycoerythrin, one of the major phycobiliproteins. InCalothrixPCC 7601, modulation of the polypeptide composition of the phycobi1isomes occurs in response to changes of the light wavelength, a phenomenon known as complementary chromatic adaptation. Under green illumination, cells synthesize phycoerythrin and its two specifically associated linker polypeptides (L35Rand L36R), while under red illumination none of these proteins are detected. Using specific probes, a single transcript (1450 nucleotide long) corresponding to the cpe genes was detected but only in green-light-grown cells, establishing the occurrence of transcriptional regulation for the expression of this operon in response to light wavelength changes. The size of this transcript excludes the possibility that the phycoerythrin-associated L35Rand L36Rcould be cotranscribed with thecpeAandcpeBgenes.