Periodontal acidification contributes to tooth pain hypersensitivity during orthodontic tooth movement
Periodontal acidification contributes to tooth pain hypersensitivity during orthodontic tooth movement
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DOI：
10.1016/j.neures.2021.11.007
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发表时间：
2022-04-07
影响因子：
2.9
通讯作者：
Shinoda, Masamichi
中科院分区：
文献类型：
--
作者：
Osada, Ayaka;Hitomi, Suzuro;Shinoda, Masamichi
牙齿移动与正畸治疗往往会导致牙痛。然而，详细的机制仍不清楚。在这里，我们研究了牙齿移动引起的牙周酸化在机械性牙痛超敏反应中的作用。在Sprague-Dawley大鼠的第一和第二磨牙之间插入弹性材料以移动牙齿。测量第一磨牙机械性缩头反射阈值和牙周龈沟pH值。分析酸敏感离子通道3（ASIC 3）在三叉神经节神经元中的表达和牙周组织中ASIC 3的磷酸化。第一磨牙刺激的机械性头回反射阈值和龈沟pH值在弹性插入后第1天下降。通过缓冲牙周酸化，这些降低恢复到假手术水平。周期性抑制ASIC 3通道活性逆转了第一磨牙刺激时降低的机械性缩头反射阈值。在弹性插入后的第1天，牙齿移动没有改变支配牙周组织的ASIC 3免疫反应性三叉神经节神经元的数量，但增加了牙周组织中磷酸化ASIC 3的水平。牙齿移动引起的牙周膜酸化导致ASIC 3磷酸化，导致机械强迫牙的机械痛过敏。
Tooth movements associated with orthodontic treatment often cause tooth pain. However, the detailed mechanism remains unclear. Here, we examined the involvement of periodontal acidification caused by tooth movement in mechanical tooth pain hypersensitivity. Elastics were inserted between the first and second molars to move the teeth in Sprague-Dawley rats. Mechanical head-withdrawal reflex threshold to first molar stimulation and the pH of the gingival sulcus around the tooth were measured. The expression of acid-sensing ion channel 3 (ASIC3) in trigeminal ganglion neurons and phosphorylation of ASIC3 in the periodontal tissue were analyzed. The mechanical head-withdrawal reflex threshold to first molar stimulation and pH in the gingival sulcus decreased on day 1 after the elastic insertion. These decreases recovered to the sham level by buffering periodontal acidification. Periodontal inhibition of ASIC3 channel activity reversed the decreased mechanical head-withdrawal reflex threshold to first molar stimulation. On day 1 after elastic insertion, the tooth movement did not change the number of ASIC3 immunoreactive trigeminal ganglion neurons innervating the periodontal tissue but increased phosphorylated-ASIC3 levels in the periodontal tissue. Periodontal acidification induced by tooth movement causes phosphorylation of ASIC3, resulting in mechanical pain hypersensitivity in mechanically forced tooth.