Electron microscopy examination of galanin-like peptide (GALP)-containing neurons in the rat hypothalamus
Electron microscopy examination of galanin-like peptide (GALP)-containing neurons in the rat hypothalamus
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DOI：
10.1016/j.regpep.2004.08.011
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发表时间：
2005-03-15
影响因子：
--
通讯作者：
Shioda, S
中科院分区：
文献类型：
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作者：
Guan, JL;Kageyama, H;Shioda, S
甘丙肽（Galanin-like peptide，GALP）是从猪下丘脑中分离到的一种新的肽。本研究应用光镜和免疫电镜技术，观察了弓状核（ARC）内含GALP神经元的超微结构和突触关系。在光学显微镜水平，观察到含GALP的神经元分布在整个ARC的喙尾，与大多数存在于后部，脑室周围区。电镜下，在含GALP神经元的胞体、树突和轴突终末可见许多免疫阳性的致密核囊泡。此外。这些神经元从免疫阴性轴突终末接受突触，在胞体上形成的突触的情况下，所述轴突终末是对称的，而在树突上形成的突触既不对称又对称。含GALP神经元的轴突终末常与免疫阴性树突形成突触。这些突触都是对称的。这些结果表明，含GALP的神经元在ARC中的生理作用是基于含GALP的神经元与GALP免疫阳性或免疫阴性神经元之间复杂的突触关系。(C)2004 Elsevier B. V.保留所有权利。
Galanin-like peptide (GALP) is a novel peptide which is isolated front the porcine hypothalamus. GALP-containing neurons are present in the arcuate nucleus (ARC), being particularly densely concentrated in medial posterior regions To observe the ultrastructure and synaptic relationships of GALP-containing neurons in the ARC, light and immunoelectron microscopy techniques were used. At the light microscope level, GALP-containing neurons were observed distributed rostrocaudally throughout the ARC, with the majority present in the posterior, periventricular zones. At the electron microscope level, many immunopositive dense-cored vesicles were evident in the perikarya, dendrites and axon terminals of the GALP-containing neurons. Furthermore. these neurons received synapses, from immunonegative axon terminals that were symmetric in the case of synapses made on perikarya, and both asymmetric and symmetric for synapses made on dendrites. Axon terminals of GALP-containing neurons often made synapses on immunonegative dendrites. Such synapses were all symmetric. Synapses were also found between axon terminals and perikarya as well as dendrites of GALP-containing neurons.These findings suggest that the physiological role of the GALP-containing neurons in the ARC is based on complex synaptic relationships between GALP-containing, neurons and either GALP-immunopositive or immunonegative neurons. (C) 2004 Elsevier B.V. All rights reserved.