Characterization of a (BALB/c x C57BL/6)F1 T cell hybridoma with double specificity: recognition of antigen in context of I-Ad and autoreactivity to I-Ab.
Characterization of a (BALB/c x C57BL/6)F1 T cell hybridoma with double specificity: recognition of antigen in context of I-Ad and autoreactivity to I-Ab.
复制标题
具有双重特异性的 (BALB/c x C57BL/6)F1 T 细胞杂交瘤的表征：在 I-Ad 背景下识别抗原以及对 I-Ab 的自身反应。
DOI：
10.1002/eji.1830140713
复制
发表时间：
1984
影响因子：
5.4
通讯作者：
Bona,CA
中科院分区：
文献类型：
--
作者：
Nagase,F;Waters,SJ;Thorbecke,GJ;Bona,CA
用匙孔血蓝蛋白（KLH）致敏的BALB/c × C57 BL/6小鼠F1淋巴结与胸腺瘤细胞BW 5147融合，获得了一株抗原特异性、产生IL 2的Lyt-1+2-T细胞杂交瘤。杂交瘤FN 13 - 21识别与BALB/c亲本抗原呈递细胞的I-Ab相关的KLH，并且在不存在抗原的情况下也对其它亲本C57 BL/6脾细胞的I-Ab产生应答。FN 13 - 21的KLH特异性反应被单克隆抗I-Ab抗体阻断，而对C57 BL/6脾细胞的反应被抗I-Ab抗体阻断。自身反应性的特异性是针对I-Ab区中编码的抗原，如用来自H-2-重组小鼠的脾细胞获得的刺激模式所示。
An antigen‐specific, IL2‐producing, Lyt‐1+2−, T cell hybridoma has been derived by the fusion of keyhole limpet hemocyanin (KLH)‐primed (BALB/c × C57BL/6)F1lymph node with BW5147 thymoma cells. The hybridoma FN13‐21 recognizes KLH in association with I‐Adof BALB/c parental antigen‐presenting cells, and also responds to I‐Abof the other parent C57BL/6 spleen cells in the absence of antigen. The KLH‐specific response of FN13‐21 is blocked by monoclonal anti‐I‐Adantibody, while the response to C57BL/6 spleen cells is blocked by anti‐I‐Abantibody. The specificity of the autoreactivity is to an antigen encoded for in the I‐Abregion, as shown by the pattern of stimulation obtained with spleen cells from H‐2‐recombinant mice.