Imaging large-scale cellular activity in spinal cord of freely behaving mice.
Imaging large-scale cellular activity in spinal cord of freely behaving mice.
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DOI：
10.1038/ncomms11450
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发表时间：
2016-04-28
影响因子：
16.6
通讯作者：
Nimmerjahn A
中科院分区：
文献类型：
--
作者：
Sekiguchi KJ;Shekhtmeyster P;Merten K;Arena A;Cook D;Hoffman E;Ngo A;Nimmerjahn A
来自皮肤中的机械感受器和伤害感受器的感觉信息在适应性和保护性运动行为中起着关键作用。到目前为止，人们对脊髓细胞类型及其活动模式如何编码这些信息知之甚少，特别是在自由行为条件下。为了能够稳定的测量神经元和神经胶质细胞的活动在行为小鼠，我们已经开发了基于双和小型化单光子显微镜的荧光成像方法。我们发现，不同的皮肤刺激激活重叠合奏的背角神经元，刺激类型和强度编码在单细胞水平。相比之下，星形胶质细胞显示出大规模的协调钙反应强烈，但不弱的感觉输入。感觉诱发的活动被麻醉剂有效地抑制。通过揭示行为条件下脊髓网络的细胞和计算逻辑，我们的方法有望更好地了解健康和异常的脊髓过程。 对小鼠脊髓中的细胞活动进行成像历来是困难的。在这里，作者在行为小鼠的脊髓中开发了细胞分辨率荧光成像方法，并报告了神经元和星形胶质细胞响应不同感觉输入的不同活动模式。
Sensory information from mechanoreceptors and nociceptors in the skin plays key roles in adaptive and protective motor behaviours. To date, very little is known about how this information is encoded by spinal cord cell types and their activity patterns, particularly under freely behaving conditions. To enable stable measurement of neuronal and glial cell activity in behaving mice, we have developed fluorescence imaging approaches based on two- and miniaturized one-photon microscopy. We show that distinct cutaneous stimuli activate overlapping ensembles of dorsal horn neurons, and that stimulus type and intensity is encoded at the single-cell level. In contrast, astrocytes show large-scale coordinated calcium responses to intense but not weak sensory inputs. Sensory-evoked activity is potently suppressed by anaesthesia. By revealing the cellular and computational logic of spinal cord networks under behaving conditions, our approach holds promise for better understanding of healthy and aberrant spinal cord processes. Imaging cellular activity in mouse spinal cord has been historically difficult. Here the authors develop cellular resolution fluorescence imaging approaches in the spinal cord of behaving mice, and report distinct activity patterns of neurons and astrocytes in response to different sensory inputs.