Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture
Antioxidant properties of water-soluble polysaccharides from Antrodia cinnamomea in submerged culture
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DOI：
10.1016/j.foodchem.2007.01.018
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Yen, Gow-Chin
中科院分区：
文献类型：
--
作者：
Tsai, Ming-Chi;Song, Tuzz-Ying;Yen, Gow-Chin
牛樟芝是我国著名的传统中药，具有抗肿瘤、抗氧化、刺激免疫等作用。研究樟芝菌丝体和发酵滤液水溶性多糖（ACSC）对过氧化氢诱导的Chang肝细胞毒性和DNA损伤的保护作用。通过单细胞凝胶电泳（彗星试验）或8-羟基脱氧鸟苷（8-OHdG）加合物的形成来评价氧化性DNA损伤。通过离子交换层析分离的多糖含有葡萄糖、木糖、半乳糖、阿拉伯糖和甘露糖。结果表明，孵育的Chang肝细胞与分离的多糖在200 μ g/mL的5小时之前，H2 O2处理（50 μ M，30分钟）显着减少DNA氧化损伤的彗星尾DNA和8-OHdG加合物的形成所检测到的89%和69%，分别。经多糖预处理的Chang肝细胞，其硫代巴比妥酸反应物质（TBARS）和细胞内活性氧（ROS）水平均显著降低（p <0.01）。此外，经多糖预处理的Chang肝细胞，其谷胱甘肽S-转移酶（GST）和GSH/GSSG比值显著升高（p < 0.01）。这些结果表明，ASCS中的多糖具有抗氧化特性，这可能涉及上调GST活性，维持正常的GSH/GSSG比例，并清除ROS。(c)2007爱思唯尔有限公司保留所有权利。
Antrodia cinnamomea, a well-known tradition Chinese medicine, possesses anti-tumor, anti-oxidation activities and stimulates the immune system. The aim of this study was to investigate the protective effect of water-soluble polysaccharides from the fermented filtrate and mycelia of Antrodia cinnamomea in submerged culture (ACSC) on hydrogen peroxide-induced cytotoxicity and DNA damage in Chang liver cells. Oxidative DNA damage was evaluated by single cell gel electrophoresis (Comet assay) or by the formation of 8-hydroxy-deoxyguanosine (8-OHdG) adducts. The polysaccharides isolated by ion-exchange chromatography contained glucose, xylose, galactose, arabinose, and mannose. The results showed that incubation of Chang liver cells with isolated polysaccharides at 200 mu g/mL for 5 h prior to H2O2 treatment (50 mu M, 30 min) significantly reduced oxidative DNA damage as detected by the formation of comet tail DNA and 8-OHdG adducts by 89% and 69%, respectively. Pre-treatment Chang liver cells with polysaccharides also reduced the levels of thiobarbituric acid reactive substances (TBARS) (p < 0.01) and intracellular reactive species (ROS) (p < 0.01) induced by H2O2- Moreover, glutathione S-transferase (GST) and the GSH/GSSG ratio were significantly increased in Chang liver cells pre-incubated with the polysaccharides (p < 0.01). These results demonstrate that polysaccharides in ASCS have antioxidant properties which may involve up-regulation of GST activity, maintenance of normal GSH/GSSG ratio, and scavenging of ROS. (c) 2007 Elsevier Ltd. All rights reserved.