RNA-Directed DNA Methylation Is Involved in Regulating Photoperiod-Sensitive Male Sterility in Rice
RNA-Directed DNA Methylation Is Involved in Regulating Photoperiod-Sensitive Male Sterility in Rice
复制标题
DOI：
10.1093/mp/sss095
复制
发表时间：
2012-11-01
期刊：
影响因子：
27.5
通讯作者：
Zhang, Qifa
中科院分区：
文献类型：
--
作者：
Ding, Jihua;Shen, Jianqiang;Zhang, Qifa
光敏核不育系是一种重要的杂交水稻种质资源。最近，我们克隆了一个控制PSMS的基因座pms3，它编码一个长的非编码RNA，称为LDMAR，是水稻植株在长日照条件下正常雄性育性所需的。PSMS水稻（农垦585）中LDMAR启动子甲基化相对于野生型（农垦58）的增加降低了LDMAR的表达，导致长日照条件下的雄性不育。在这项研究中，我们确定了一个名为Psi LDMAR的siRNA在LDMAR启动子区，在农垦585中比农垦58中更丰富。我们表明Psi LDMAR可能来源于AK111270，AK111270是从LDMAR启动子的有义链获得的转录物，其3 '端与LDMAR的5'端具有110个碱基的重叠。AK111270在农垦585中的过表达极大地富集了Psi LDMAR，这诱导了LDMAR启动子中RNA指导的DNA甲基化，并抑制了LDMAR的表达。农垦585的LDMAR降低改变了育性恢复的临界日长，推迟了短日条件下的育性恢复。这一结果增加了我们对PSMS分子机制的理解。
Photoperiod-sensitive male sterility (PSMS) is a valuable germplasm for hybrid rice breeding. Recently, we cloned pms3, a locus controlling PSMS, which encodes a long non-coding RNA called LDMAR required for normal male fertility of the rice plant under long-day conditions. Increased methylation in the promoter of LDMAR in the PSMS rice (Nongken 585) relative to the wild-type (Nongken 58) reduced expression of LDMAR leading to male sterility under long-day conditions. In this study, we identified a siRNA named Psi LDMAR in the LDMAR promoter region that was more abundant in Nongken 585 than in Nongken 58. We showed that Psi LDMAR was likely derived from AK111270, a transcript obtained from the sense strand of the LDMAR promoter with its 3'-end having a 110-base overlap with the 5'-end of LDMAR. Overexpressing AK111270 in Nongken 585 greatly enriched Psi LDMAR, which induced RNA-directed DNA methylation in the LDMAR promoter and repressed the expression of LDMAR. Reduction of LDMAR in Nongken 585 changed the critical day length for fertility recovery and delayed the fertility recovery under short-day conditions. This result added to our understanding of the molecular mechanism for PSMS.