MYB Superfamily in Brassica napus: Evidence for Hormone-Mediated Expression Profiles, Large Expansion, and Functions in Root Hair Development
MYB Superfamily in Brassica napus: Evidence for Hormone-Mediated Expression Profiles, Large Expansion, and Functions in Root Hair Development
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甘蓝型油菜中的 MYB 超家族：激素介导的表达谱、大扩展和根毛发育功能的证据
DOI：
10.3390/biom10060875
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发表时间：
2020-06-01
期刊：
影响因子：
5.5
通讯作者：
Du, Hai
中科院分区：
文献类型：
--
作者：
Li, Pengfeng;Wen, Jing;Du, Hai
MYB蛋白参与植物多种重要的生物过程。本研究从异源四倍体甘蓝型油菜中获得了MYB超家族，其中包含227个MYB相关基因（BnMYBR/Bn1R-MYB）、429个R2R3-MYB （Bn2R-MYB）、22个R1R2R3-MYB （Bn3R-MYB）和2个R1R2R2R1/2-MYB （Bn4R-MYB）。系统发育分析将bn2r - myb分为43个亚家族，bnmybr分为5个亚家族。bn2r - myb和bnmybr亚家族的序列特征和外显子/内含子结构高度保守。整个超家族不均匀分布在19条染色体上，在甘蓝型油菜中扩展不平衡。甘蓝与rapa的异源多倍体性是其后代油菜扩增的主要原因，在后代油菜中rapa衍生基因的保留较多。对9种芸苔科植物和7种非芸苔科植物的2R-MYB蛋白进行系统发育分析，鉴定出5个芸苔科植物特异性亚科和5个芸苔科植物缺乏的亚科，为2R-MYB基因家族在被子植物多样化过程中的适应性进化提供了例证。在CaMV35S病毒和/或原生启动子的控制下，4个Bn2R-MYBs的异位表达可以挽救突变植物拟南芥的少根毛表型，证明了S15亚家族的2R-MYBs在根毛发育中的保守负作用。rna测序数据显示，在不同激素处理下，Bn2R-MYBs和BnMYBRs在根中具有不同的转录谱。我们的发现为进一步研究甘蓝型油菜MYB基因的功能特征提供了有价值的信息。
MYB proteins are involved in diverse important biological processes in plants. Herein, we obtained the MYB superfamily from the allotetraploid Brassica napus, which contains 227 MYB-related (BnMYBR/Bn1R-MYB), 429 R2R3-MYB (Bn2R-MYB), 22 R1R2R3-MYB (Bn3R-MYB), and two R1R2R2R1/2-MYB (Bn4R-MYB) genes. Phylogenetic analysis classified the Bn2R-MYBs into 43 subfamilies, and the BnMYBRs into five subfamilies. Sequence characteristics and exon/intron structures within each subfamily of the Bn2R-MYBs and BnMYBRs were highly conserved. The whole superfamily was unevenly distributed on 19 chromosomes and underwent unbalanced expansion in B. napus. Allopolyploidy between B. oleracea and B. rapa mainly contributed to the expansion in their descendent B. napus, in which B. rapa-derived genes were more retained. Comparative phylogenetic analysis of 2R-MYB proteins from nine Brassicaceae and seven non-Brassicaceae species identified five Brassicaceae-specific subfamilies and five subfamilies that are lacking from the examined Brassicaceae species, which provided an example for the adaptive evolution of the 2R-MYB gene family alongside angiosperm diversification. Ectopic expression of four Bn2R-MYBs under the control of the viral CaMV35S and/or native promoters could rescue the lesser root hair phenotype of the Arabidopsis thaliana wer mutant plants, proving the conserved negative roles of the 2R-MYBs of the S15 subfamily in root hair development. RNA-sequencing data revealed that the Bn2R-MYBs and BnMYBRs had diverse transcript profiles in roots in response to the treatments with various hormones. Our findings provide valuable information for further functional characterizations of B. napus MYB genes.