Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.
Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.
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DOI：
10.1016/j.crmeth.2023.100506
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发表时间：
2023-06-26
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影响因子：
--
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Brain functions are accomplished by polysynaptic circuits formed by neurons wired together through multiple orders of synaptic connections. Polysynaptic connectivity has been difficult to examine due to a lack of methods of continuously tracing the pathways in a controlled manner. Here, we demonstrate directed, stepwise retrograde polysynaptic tracing by inducible reconstitution of replication-deficient trans-neuronal pseudorabies virus (PRVΔIE) in the brain. Furthermore, PRVΔIE replication can be temporally restricted to minimize its neurotoxicity. With this tool, we delineate a wiring diagram between the hippocampus and striatum—two major brain systems for learning, memory, and navigation—that consists of projections from specific hippocampal domains to specific striatal areas via distinct intermediate brain regions. Therefore, this inducible PRVΔIE system provides a tool for dissecting polysynaptic circuits underlying complex brain functions. Efficient trans-complementation of replication-deficient pseudorabies virus Stepwise tracing of polysynaptic circuits in a pre-specified direction Minimizes neuronal toxicity by temporally restricting viral replication Delineates the polysynaptic wiring diagram between hippocampus and striatum The brain is a complex network formed by neurons connected to each other sequentially. To understand its functions, we need to dissect the polysynaptic connectivity of this network. This study develops a tool for tracing the connections in polysynaptic circuits. In the brain, information is transmitted and processed by polysynaptic circuits formed by neurons wired together sequentially. Polysynaptic connectivity has been difficult to examine. Here, Du et al. demonstrate that inducible trans-complementation of replication-deficient retrograde transneuronal pseudorabies virus (PRVΔIE) allows continuous tracing of neuronal pathways in a directed and stepwise manner.
影响因子：
3.7
作者：
Kratchmarov R;Taylor MP;Enquist LW
通讯作者：
Enquist LW