Relative quantitative expression of hypoxia-inducible factor 1 alpha messenger ribonucleic acid in recurrent craniopharyngiomas
Relative quantitative expression of hypoxia-inducible factor 1 alpha messenger ribonucleic acid in recurrent craniopharyngiomas
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复发性颅咽管瘤缺氧诱导因子1α信使核糖核酸的相对定量表达
DOI：
10.4103/0028-3886.128291
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发表时间：
2014-01-01
期刊：
影响因子：
2.7
通讯作者：
Xu, Jianguo
中科院分区：
文献类型：
--
作者：
Liu, Hao;Liu, Zhiyong;Xu, Jianguo
背景：颅咽管瘤是一种良性肿瘤，但复发率高，预后差。其高复发率的病理机制尚不清楚。目的：我们假设颅咽管瘤内存在缺氧微环境，缺氧诱导因子-1 α（HIF-1 α）及其相关基因在复发性颅咽管瘤中大量表达。材料和方法：共19例颅咽管瘤已确定。采用真实的时间反转录聚合酶链反应（RT-PCR）检测颅咽管瘤组织中HIF-1 α、血管内皮生长因子（VEGF）和碳酸酐酶9（CA 9）信使核糖核酸（mRNA）的相对定量表达。结果：复发性颅咽管瘤中HIF-1 α和VEGF mRNA表达明显上调。HIF-1 α的平均表达水平（复发性颅咽管瘤与非复发性颅咽管瘤相比，标准化为β-肌动蛋白表达）为3.09 vs 0.75（P = 0.001），VEGF为1.07 vs 0.32（P = 0.000），CA 9为1.21 vs 1.93（P = 0.503）。在颅咽管瘤中，VEGF与HIF-1 α的表达呈显著相关（r = 0.836，P = 0.000）。结论：颅咽管瘤内存在缺氧微环境。因此，阻止肿瘤细胞对低氧环境的适应可能是防止颅咽管瘤复发的有效途径。
Background: Craniopharyngioma is a benign tumor, but recurrences are common and prognosis is poor. The pathologic mechanism underlying the high recurrence is still unknown. Aims: We hypothesized that there are hypoxic microenvironments within craniopharyngiomas and hypoxia inducible factor-1 alpha (HIF-1 alpha) and its related genes are largely expressed in recurrent craniopharyngiomas. Materials and Methods: A total of 19 patients with craniopharyngiomas have been identified. The relative quantitative expressions of HIF-1 alpha, vascular endothelial growth factor (VEGF) and carbonic adhydrase 9 (CA9) messenger ribonucleic acid (mRNA) of craniopharyngioma tissues were detected by real time reverse transcription polymerase chain reaction. Results: HIF-1 alpha and VEGF mRNA was significantly up-regulated in recurrent craniopharyngiomas. Mean expression levels (recurrent craniopharyngiomas compared with non-recurrent craniopharyngiomas, as normalized to expression of beta-actin) were 3.09 versus 0.75 (P = 0.001) for HIF-1 alpha, 1.07 versus 0.32 (P = 0.000) for VEGF, 1.21 versus 1.93 (P = 0.503) for CA9. In craniopharyngiomas, the expression of VEGF showed a significant correlation with HIF-1 alpha (r = 0.836, P = 0.000). Conclusion: There were hypoxic microenvironments within craniopharyngiomas. Therefore, preventing the tumor cells from adapting to the hypoxic conditions may be an effective way to obviate the relapse of craniopharyngioma.