THIOL AND DISULPHIDE CONTENTS OF HEN OVALBUMIN - C-TERMINAL SEQUENCE AND LOCATION OF DISULPHIDE BOND
THIOL AND DISULPHIDE CONTENTS OF HEN OVALBUMIN - C-TERMINAL SEQUENCE AND LOCATION OF DISULPHIDE BOND
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DOI：
10.1042/bj1160555
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发表时间：
1970-01-01
影响因子：
4.1
通讯作者：
FOTHERGI.JE
中科院分区：
文献类型：
--
作者：
FOTHERGI.LA;FOTHERGI.JE
1.用氯汞苯甲酸盐滴定法、过甲酸氧化后半胱氨酸含量测定法、放射性碘乙酸摄取量测定法、S-氨乙基半胱氨酸与乙烯亚胺反应后测定法，研究了鸡卵清蛋白中巯基和二硫键的含量。结果表明，卵清蛋白含有6个半胱氨酸残基。有无还原剂的实验表明，有4个巯基和1个二硫键。2.一种肽含有等摩尔量的S-羧甲基-半胱氨酸，丝氨酸，缬氨酸和脯氨酸，但没有赖氨酸或精氨酸，通过放射性标记的半胱氨酸残基与碘[14 C]乙酸，然后通过电泳和层析分离的胰蛋白酶的胰蛋白酶。结果表明，卵清蛋白C端序列为-Cys-Val-Ser-Pro。3.二硫键的位置进行了研究，通过使用双标记技术。结果表明，二硫键的一端位于该C端肽段。
1. The thiol and disulphide contents of hen ovalbumin were investigated byp-chloromercuribenzoate titration, by determination of cysteic acid content after performic acid oxidation, by measurement of uptake of radioactive iodoacetic acid, and by assay ofS-aminoethylcysteine after reaction with ethyleneimine. All results showed that ovalbumin had 6 half-cystine residues. Experiments with and without reducing agents demonstrated that there were 4 thiol groups and 1 disulphide bond. 2. A peptide containing equimolar amounts ofS-carboxymethyl-cysteine, serine, valine and proline, but no lysine or arginine, was obtained by radioactive labelling of the cysteine residues with iodo[14C]acetic acid followed by electrophoretic and chromatographic separation of tryptic digests. It was concluded that theC-terminal sequence of ovalbumin is -Cys-Val-Ser-Pro. 3. The location of the disulphide bond was studied by using a double-labelling technique. It was shown that one end of the disulphide was located in thisC-terminal peptide.