A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections
A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections
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DOI：
10.1016/j.jfma.2011.11.029
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发表时间：
2012-12-01
影响因子：
3.2
通讯作者：
Huang, Li-Min
中科院分区：
文献类型：
--
作者：
Lu, Chun-Yi;Chang, Luan-Yin;Huang, Li-Min
背景/目的：准确和及时的诊断对于流感的临床管理至关重要。方法：选择2009年甲型H1N1流感患者咽拭子标本90份，采用酶联免疫吸附试验（ELISA）检测2009年甲型H1N1流感病毒。使用逆转录聚合酶链反应作为金标准，总体灵敏度（0.57）高于QuickVue Influenza A+B Test（0.43）。使用选定的样品集，ELISA的特异性为1.0。结论：ELISA法是一种简便易行、特异性强、敏感性高的甲型H1N1流感病毒感染的诊断方法。病毒载量和特异性之间存在很强的相关性。版权所有（C）2012，爱思唯尔台湾有限公司及台湾医学会. All rights reserved.
Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples.Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested.Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively.Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.