Evolution of the leucine-rich repeat receptor-like protein kinase gene family: Ancestral copy number and functional divergence of BAM1 and BAM2 in Brassicaceae
Evolution of the leucine-rich repeat receptor-like protein kinase gene family: Ancestral copy number and functional divergence of BAM1 and BAM2 in Brassicaceae
复制标题
富含亮氨酸重复受体样蛋白激酶基因家族的进化：十字花科中 BAM1 和 BAM2 的祖先拷贝数和功能差异
DOI：
10.1111/jse.12206
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发表时间：
2016
影响因子：
3.7
通讯作者：
Ma Hong
中科院分区：
文献类型：
--
作者：
Li Hao;Shi Qian;Zhang Zai-Bao;Zeng Li-Ping;Qi Ji;Ma Hong
Gene duplication allows for functional divergence and innovation that provide selective advantages. However, in flowering plants genetic studies have revealed that single‐gene mutations affecting one of two or more closely related paralogs often fail to cause detectable morphological defects, suggesting functional redundancy. Flowering plants have hundreds of genes encoding leucine‐rich repeat receptor‐like protein kinases (LRR‐RLKs), several of which play important roles in anther development, but little is known about their evolutionary history and possible functional divergence. We investigated the evolutionary relationship of the LRR‐RLK gene family by phylogenetic analysis and found that these closely related paralogs resulted from multiple duplication events, such as the one resulting inBAM1andBAM2. We further used quantitative real‐time reverse transcription–polymerase chain reaction to verify gene expression changes in immature anthers from thebam1/bam2single and double mutants compared with wild type, providing strong evidence that theBAM1andBAM2genes have evolved different functions, with differential effects on anther gene expression. Moreover, careful examination of anther development inbam1andbam2single mutants revealed previously unrecognized extra cell division in tapetum cell layers. Thus our results from phylogenetic, molecular, and morphological analyses uncover sequence and functional differences between paralogs whose single mutants lack obvious fertility defects, effectively revealing functional divergence of duplicate genes.
