Inhibitory effect of naringenin chalcone on inflammatory changes in the interaction between adipocytes and macrophages
Inhibitory effect of naringenin chalcone on inflammatory changes in the interaction between adipocytes and macrophages
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DOI：
10.1016/j.lfs.2007.09.001
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发表时间：
2007-09-29
期刊：
影响因子：
6.1
通讯作者：
Kawada, Teruo
中科院分区：
文献类型：
--
作者：
Hirai, Shizuka;Kim, Young-Ii;Kawada, Teruo
肥胖脂肪组织的特征在于巨噬细胞的浸润增强。认为脂肪细胞和巨噬细胞之间的涉及单核细胞趋化蛋白（MCP）-1和肿瘤坏死因子（TNF）-α的旁分泌环建立了恶性循环，其增强了肥胖脂肪组织中的炎症变化和胰岛素抵抗。多酚广泛分布于水果和蔬菜中，可以作为抗氧化剂，其中一些也被报道具有抗炎特性。番茄是世界上最受欢迎和消费最广泛的蔬菜作物之一，它也含有许多黄酮类化合物，主要是柚皮素查尔酮。我们研究了黄酮类化合物，包括柚皮素查耳酮，对脂多糖（LPS）刺激的巨噬细胞中促炎介质的产生以及脂肪细胞和巨噬细胞之间的相互作用的影响。柚皮素查耳酮以剂量依赖性方式抑制LPS刺激的RAW 264巨噬细胞产生TNF-α、MCP-1和一氧化氮（NO）。与对照培养物相比，3 T3-L1脂肪细胞和RAW 264巨噬细胞的共培养显著增强了TNF-α、MCP-1和NO的产生;然而，用柚皮素查耳酮剂量依赖性地抑制了这些促炎介质的产生。这些结果表明，柚皮素查耳酮通过抑制脂肪细胞和巨噬细胞之间相互作用中促炎细胞因子的产生而表现出抗炎特性。柚皮素查耳酮可能有助于改善肥胖脂肪组织的炎症变化。(c)2007爱思唯尔公司All rights reserved.
Obese adipose tissue is characterized by an enhanced infiltration of macrophages. It is considered that the paracrine loop involving monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor (TNF)-alpha between adipocytes and macrophages establishes a vicious cycle that augments the inflammatory changes and insulin resistance in obese adipose tissue. Polyphenols, which are widely distributed in fruit and vegetables, can act as antioxidants and some of them are also reported to have anti-inflammatory properties. Tomato is one of the most popular and extensively consumed vegetable crops worldwide, which also contains many flavonoids, mainly naringenin chalcone. We investigated the effect of flavonoids, including naringenin chalcone, on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated macrophages and in the interaction between adipocytes and macrophages. Naringenin chalcone inhibited the production of TNF-alpha, MCP-1, and nitric oxide (NO) by LPS-stimulated RAW 264 macrophages in a dose-dependent manner. Coculture of 3T3-L1 adipocytes and RAW 264 macrophages markedly enhanced the production of TNF-alpha, MCP-1, and NO compared with the control cultures; however, treatment with naringenin chalcone dose-dependently inhibited the production of these proinflammatory mediators. These results indicate that naringenin chalcone exhibits anti-inflammatory properties by inhibiting the production of proinflammatory cytokines in the interaction between adipocytes and macrophages. Naringenin chalcone may be useful for ameliorating the inflammatory changes in obese adipose tissue. (c) 2007 Elsevier Inc. All rights reserved.