Interactions in biofilms of Lactococcus lactis ssp cremoris and Pseudomonas fluorescens cultured in cold UHT milk
Interactions in biofilms of Lactococcus lactis ssp cremoris and Pseudomonas fluorescens cultured in cold UHT milk
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DOI：
10.3168/jds.s0022-0302(05)73102-7
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发表时间：
2005-12-01
影响因子：
3.5
通讯作者：
SanJose, C
中科院分区：
文献类型：
--
作者：
Kives, J;Guadarrama, D;SanJose, C
三种乳酸乳球菌。从冷冻散装原料奶中分离出的cremoris分离物与一种已知的嗜冷性奶牛荧光假单胞菌菌株在脱脂UHT牛奶中在7℃下单独培养72小时，以确定浮游和生物膜阶段的相互影响。每个培养伙伴的两级接种量合并。原合作和共生现象均发生在生物膜期。相互作用的类型和强度取决于乳球菌菌株和每个伙伴的细胞密度。观察到荧光假单胞菌的最大附着增强约为100倍，乳酸乳杆菌的最大附着增强约为20,000倍。激光共聚焦扫描显微镜图像显示假单胞菌在协同生物膜中捕获乳酸球菌细胞的致密团块。
Three Lactococcus lactis ssp. cremoris isolates from refrigerated bulk raw milk were cultured separately and in association with a known psychrotrophic dairy Pseudomonas fluorescens strain, in skim UHT milk for 72 h at 7 degrees C, to determine mutual influences in both the planktonic and biofilm phases. Two levels of inoculum of each culture partner were combined. Protocooperation and commensalism cases were found, all of them in the biofilm phase. Type and intensity of the interactions depended on Lactococcus strain and on the cell density of each partner. Maximum enhancement of attachment was observed to be approximately 100-fold for P. fluorescens and 20,000-fold for one of the L. lactis strains. Confocal scanning laser microscopy images show compact masses of Pseudomonas trapping lactococci cells in cooperative biofilms.