STUDIES ON THE EFFECTS OF THE NARCOTIC ALKALOIDS, COCAINE, MORPHINE, AND CODEINE ON NONENZYMATIC LIPID-PEROXIDATION IN RAT-BRAIN MITOCHONDRIA
STUDIES ON THE EFFECTS OF THE NARCOTIC ALKALOIDS, COCAINE, MORPHINE, AND CODEINE ON NONENZYMATIC LIPID-PEROXIDATION IN RAT-BRAIN MITOCHONDRIA
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DOI：
10.1016/0885-4505(87)90035-1
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发表时间：
1987-04-01
期刊：
影响因子：
--
通讯作者：
RATTY, AK
中科院分区：
文献类型：
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作者：
DAS, NP;RATTY, AK
本文提供了麻醉生物碱、盐酸可卡因、硫酸吗啡和磷酸可待因对大鼠脑线粒体非酶脂质过氧化作用的研究证据。这些细胞器富含多不饱和脂肪酸，因此容易受到氧化攻击。脂质过氧化主要通过测定丙二醛（MDA）产生的程度以及反应过程中荧光产物的形成来衡量。我们发现，在没有诱导剂（1.0 mM 抗坏血酸或 0.1 mM 硫酸亚铁）的情况下，硫酸吗啡会降低荧光，而其他两种生物碱对脂质过氧化没有影响。硫酸吗啡的明显抗氧化性质还体现在其对诱导和非诱导 MDA 产生的影响中，在没有任何诱导剂的情况下，盐酸可卡因和磷酸可待因均可刺激 MDA 产生约 20%。本文还尝试绘制鸦片生物碱抗氧化作用的构效关系，我们推测这是由于生物碱分子的螯合能力。
This paper presents evidence of studies on the effects of the narcotic alkaloids, cocaine hydrochloride, morphine sulfate, and codeine phosphate, on nonenzymatic lipid peroxidation in rat brain mitochondria. These organelles abound in polyunsaturated fatty acids and are thus susceptible to oxidative attack. Lipid peroxidation was indexed mainly by assaying the extent of malonaldehyde (MDA) production and also the formation of fluorescent products in the course of the reaction. We found that morphine sulfate lowered fluorescence while the other two alkaloids showed no effect on lipid peroxidation in the absence of the inducers, 1.0 mM ascorbic acid or 0.1 mM ferrous sulfate. The apparent antioxidative nature of morphine sulfate was also observed in its effects on induced and noninduced MDA production, both cocaine hydrochloride and codeine phosphate stimulated MDA production by about 20% in the absence of any inducers. This paper also attempts to draw a structure-activity relationship for the antioxidative action of opium alkaloids, which we postulated to be due to the chelating capability of the alkaloid molecule.