Use of a chitosan–cadmium polymer as a redox hybridization indicator for CaMV35S promoter gene detection
Use of a chitosan–cadmium polymer as a redox hybridization indicator for CaMV35S promoter gene detection
复制标题
DOI：
10.1177/0883911512440537
复制
发表时间：
2012-05
影响因子：
1.7
通讯作者：
Qingxiang Wang;Jiancong Ni;F. Gao;Bin Zhang;Shunxing Li;K. Jiao
中科院分区：
文献类型：
--
作者：
Qingxiang Wang;Jiancong Ni;F. Gao;Bin Zhang;Shunxing Li;K. Jiao
合成了水溶性壳聚糖配位镉聚合物，并研究了其对单链和双链DNA的识别能力。均相溶液中的电化学分析表明，由于与两种 DNA 的相互作用不同，壳聚糖配位的镉聚合物与单链 DNA 的结合比与双链 DNA 的结合强 48 倍。表面电化学吸附的壳聚糖配位镉聚合物分子很容易通过冲洗从双链DNA修饰电极上去除，但不能从单链DNA修饰电极上去除。基于这一事实，该聚合物被用作DNA杂交检测的氧化还原指示剂，因为该聚合物能够以低背景干扰识别互补、非互补和碱基错配序列。目标DNA序列定量范围为5.0×10-9至5.0×10-7 M，检测限为4.2×10-9 M。对一种转基因大豆真实样品的DNA进行聚合酶链式反应扩增，检测结果也令人满意。
A water-soluble chitosan-coordinated cadmium polymer was synthesized, and its recognition of single-stranded and double-stranded DNA was investigated. The electrochemical analysis in the homogeneous solutions revealed that the chitosan-coordinated cadmium polymer association with a single stranded DNA was 48 times greater than with double-stranded DNA due to the different interaction with the two kinds of DNA. The surface-based electrochemically absorbed chitosan-coordinated cadmium polymer molecules were easily removed from the double-stranded DNA-modified electrode via rinsing but could not be removed from a single-stranded DNA-modified electrode. Based on this fact, the polymer was utilized as a redox indicator for DNA hybridization detection, as the polymer was able to recognize complementary, noncomplementary, and base-mismatched sequences with a low background interference. The target DNA sequence was quantified from 5.0 × 10-9 to 5.0 × 10-7 M with a detection limit of 4.2 × 10-9 M. The polymerase chain reaction amplification of DNA from the real sample of a kind of transgenically modified soybeans was also detected satisfactorily.