Resistance gene cloning from a wild crop relative by sequence capture and association genetics
Resistance gene cloning from a wild crop relative by sequence capture and association genetics
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DOI：
10.1038/s41587-018-0007-9
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发表时间：
2019-02-01
影响因子：
46.9
通讯作者：
Wulff, Brande B. H.
中科院分区：
文献类型：
--
作者：
Arora, Sanu;Steuernagel, Burkhard;Wulff, Brande B. H.
野生近缘植物抗病基因可用于培育农作物的广谱抗病性。我们将关联遗传学与R基因富集测序（AgRenSeq）相结合，利用野生二倍体小麦的全基因组变异，快速克隆了4个抗秆锈基因。AgRenSeq能够在具有多样种质的任何作物中克隆R基因。
Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.