Modification of multistage skin carcinogenesis in mice.
Modification of multistage skin carcinogenesis in mice.
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小鼠多阶段皮肤癌发生的改变。
DOI：
10.1159/000419252
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
DiGiovanni,J
中科院分区：
文献类型：
--
作者：
DiGiovanni,J
Chemical carcinogenesis in mice can be characterized as either multi-stage or complete [1, 2]. Complete carcinogenesis experimental protocols involve the administration of a single large dose or repeated applications of smaller doses of a carcinogen to an experimental animal. In the mouse skin tumorigenesis system, multiple papillomas and carcinomas can be produced on the backs of mice following a single application of as little as 600-800 nmol of 7, 12-dimethylbenz (a) anthracene (DMBA)[3, 4]. Presumably, both the initiating and promoting components are present under these experimental conditions. The induction of mouse skin tumors can also be accomplished by using a multistage model that involves the processes defined operationally and mechanistically as initiation and promotion [1, 2 and refs therein]. Initiation is accomplished by topical application of a single small dose of a skin carcinogen, such as DMBA, and is essentially irreversible. An initiating dose of a carcinogen per se will not lead to the development of visible tumors. Visible tumors will result only following prolonged and repeated applications of a tumor promoter, such as croton oil or its most active constituent 12-O-tetradecanoylphorbol-13-acetate (TPA), to the initiated skin [1, 2]. This protocol, now used in most multistage carcinogenesis studies in mouse skin, was first described by Mottram [5]. In these early experiments, Mottram elicited skin tumors by treating the backs of mice with a single, subcarcinogenic dose of benzo (a) pyrene (B (a) P) and followed this with repeated applications of croton oil, obtained from the seeds of Croton tiglium.
