States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges
States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges
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DOI：
10.1002/ppap.200800036
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发表时间：
2008-08
影响因子：
3.5
通讯作者：
H. Yasuda;Mai Hashimoto;M. Rahman;K. Takashima;A. Mizuno
中科院分区：
文献类型：
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作者：
H. Yasuda;Mai Hashimoto;M. Rahman;K. Takashima;A. Mizuno
常压DBD已应用于大肠杆菌和噬菌体-2的湿态。经DBD处理后，大肠杆菌和2噬菌体均立即失活。在失活过程中监测不同生物组分的状态。仅观察到蛋白质，DNA和膜的轻微和缓慢降解，仅在灭菌完成后才看到显着的降解。将重组GFP引入大肠杆菌细胞的分析证明，DBD具有显著的蛋白质变性活性，而不影响肽键。在DBD应用的早期阶段，可以看到蛋白质的不可逆变性，可能在细菌和噬菌体的失活中发挥核心作用。
Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage-2. Upon DBD treatment, both E.coli and 2phage were immediately inactivated. The states of different biological components were monitored during the course of inactivation. Only minor and slow degradation of proteins, DNA, and membranes was observed, a remarkable degradation was seen only after the completion of sterilization. Analysis of GFP recombinantly introduced into E.coli cells proved that the DBD has a prominent protein denaturation activity without affecting peptide bonds. The irreversible denaturation of proteins, seen in the early stage of DBD application, may play a central role in inactivation of both the bacteria and bacteriophages.