Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases
Measurement of TFF3 mRNA in aspirates from thyroid nodules using mesh filtration: The first clinical trial in 130 cases
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DOI：
10.1507/endocrj.ej12-0077
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发表时间：
2012-07-20
影响因子：
2
通讯作者：
Miyauchi, Akira
中科院分区：
文献类型：
--
作者：
Yamada, Hiroya;Takano, Toru;Miyauchi, Akira
测量抽吸物中甲状腺肿瘤细胞的基因表达水平很困难，因为它受到外周血细胞或浸润淋巴细胞的干扰。在这项研究中，我们建立了一种通过网状过滤有效地将甲状腺肿瘤细胞与血细胞分离的新方法。使用 LGALS3 mRNA 作为内部对照（T/G 比），评估 148 例术前甲状腺抽吸物中三叶因子 3 (TFF3) mRNA 的表达水平。高、中、低样品的 T/G 比值的批内变异系数 (CV) 分别为 6.5%、2.5% 和 9.7%，高、中、低样品的批间 CV 分别为 27.7%、21.9% 和 38.2%。 T/G 比和细胞学方面的非诊断样本分别为 12.2% 和 16.9%。我们没有观察到血细胞污染对数据造成干扰。在这些患者中，有 12 名患者接受了两次以上的重复抽吸。除了两种情况外，我们没有观察到明显的日常变化。所有 13 例术前细胞学诊断为恶性的抽吸物均显示极低的 T/G 比值，而 93 例细胞学诊断为良性的抽吸物显示 T/G 比值差异极大，其中 21.5% 的 T/G 比值低于截止值。 11例接受了手术。所有T/G比值低的结节经病理诊断均诊断为乳头状癌。然而，手术后诊断为滤泡性腺瘤的一个结节显示出较高的 T/G 比值。我们目前的方法可能是一种有前途的术前测试，用于测量甲状腺抽吸物中的 mRNA。
Measurement of gene expression levels in thyroid tumor cells in aspirates was difficult because it is interfered with peripheral blood cells or infiltrating lymphocytes. In this study, we established a novel method to separate thyroid tumor cells from blood cells efficiently with mesh filtration. The expression level of trefoil factor 3 (TFF3) mRNA was estimated using LGALS3 mRNA as an internal control (T/G ratio) in 148 preoperative thyroid aspirates. Intra-assay coefficients of variation (CV) of T/G ratio for high, moderate, and low samples were 6.5%, 2.5%, and 9.7%, respectively, and inter-assay CV for high, moderate, and low samples were 27.7%, 21.9%, and 38.2%, respectively. Nondiagnostic samples in terms of T/G ratio and cytology were 12.2% and 16.9%, respectively. We observed no interference with the data by contaminating blood cells. Among these patients, 12 patients received more than two repeated aspirations. We did not observe a marked day-to-day variation except in two cases. All 13 preoperative aspirates diagnosed as malignant by cytology showed an extremely low T/G ratio, whereas 93 aspirates diagnosed as benign by cytology showed extremely varied T/G ratios and 21.5% of them showed a T/G ratio below the cut-off value. Eleven cases underwent surgery. All nodules showing a low T/G ratio were diagnosed as papillary carcinoma by pathological diagnosis. However, one nodule diagnosed as follicular adenoma after surgery showed a high T/G ratio. Our present method may be a promising preoperative test for measuring mRNAs in thyroid aspirates.