SENSING STRUCTURAL INTERMEDIATES IN BACTERIAL FLAGELLAR ASSEMBLY BY EXPORT OF A NEGATIVE REGULATOR
SENSING STRUCTURAL INTERMEDIATES IN BACTERIAL FLAGELLAR ASSEMBLY BY EXPORT OF A NEGATIVE REGULATOR
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DOI：
10.1126/science.8235660
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发表时间：
1993-11-19
期刊：
影响因子：
56.9
通讯作者：
KARLINSEY, JE
中科院分区：
文献类型：
--
作者：
HUGHES, KT;GILLEN, KL;KARLINSEY, JE
研究了调节蛋白感知细菌鞭毛结构完整性的能力。为了响应有缺陷的钩-基体复合物，抗σ（28）FlgM蛋白抑制鞭毛蛋白转录。在具有功能性钩-基体复合体的细胞中，鞭毛蛋白基因正常转录，FlgM蛋白被排出到生长培养基中。在具有缺陷钩-基体结构的菌株中，FlgM不存在于培养基中。在携带FlgM-LacZ蛋白融合体的菌株中，培养基中鞭毛蛋白的存在显著减少，表明融合体阻断了鞭毛输出装置。这些结果表明，FlgM蛋白评估鞭毛钩基体复合物的完整性本身是一个由鞭毛特异性输出装置输出的基板。
The ability of a regulatory protein to sense the integrity of the bacterial flagellar structure was investigated. In response to a defective hook-basal body complex, the anti-sigma(28) FlgM protein inhibits flagellin transcription. In cells with a functional hook-basal body complex, the flagellin genes are transcribed normally and the FlgM protein is expelled into the growth medium. In strains with a defective hook-basal body structure, FlgM is absent from the media. The presence of flagellin protein in the media is substantially reduced in strains carrying a FlgM-LacZ protein fusion, suggesting that the fusion is blocking the flagellar export apparatus. These results suggest that the FlgM protein assesses the integrity of the flagellar hook-basal body complex by itself being a substrate for export by the flagellar-specific export apparatus.