SYNTHESIS OF FLUORESCENT, MONODISPERSE, COLLOIDAL SILICA PARTICLES
SYNTHESIS OF FLUORESCENT, MONODISPERSE, COLLOIDAL SILICA PARTICLES
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DOI：
10.1006/jcis.1993.1306
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发表时间：
1993-08-01
影响因子：
9.9
通讯作者：
RICKA, J
中科院分区：
文献类型：
--
作者：
NYFFENEGGER, R;QUELLET, C;RICKA, J
描述了在甲硅烷基化荧光素衍生物存在下通过四甲氧基硅烷（TMOS）的受控水解和缩聚制备胶体荧光标记的二氧化硅颗粒。使用晶种生长技术获得平均半径为80 nm的单分散颗粒和标准偏差低于8%的窄尺寸分布，而均质成核产生具有双峰尺寸分布的颗粒。标记的表观程度范围为5 × 10 ~（-8）~ 2 × 10 ~（-6）mol当量的荧光素/g二氧化硅，这取决于标记程序。
The preparation of colloidal, fluorescently labeled silica particles by controlled hydrolysis and polycondensation of tetramethoxysilane (TMOS) in the presence of a silylated fluorescein derivative is described. Monodisperse particles with average radii of 80 nm and a narrow size distribution with a standard deviation lower than 8% are obtained using the seeded growth technique, whereas homogeneous nucleation yields particles with a bimodal size distribution. The apparent degree of labeling ranges from 5 × 10-8to 2 × 10-6mol equivalent of fluorescein per gram of silica, depending on the labeling procedure.