Evidence that Ha-Ras mediates two distinguishable intracellular signals activated by v-Src.
Evidence that Ha-Ras mediates two distinguishable intracellular signals activated by v-Src.
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DOI：
10.1016/s0021-9258(19)37090-5
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发表时间：
1992-09
期刊：
影响因子：
--
通讯作者：
S. Qureshi;K. Alexandropoulos;M. Rim;C. Joseph;J. Bruder;U. Rapp;D. A. Foster
中科院分区：
文献类型：
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作者：
S. Qureshi;K. Alexandropoulos;M. Rim;C. Joseph;J. Bruder;U. Rapp;D. A. Foster
v-Src 通过两种不同的细胞内信号传导机制，在 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 反应元件 (TRE) 和血清反应元件 (SRE) 的控制下激活启动子。 v-Src 对 TRE 和 SRE 介导的基因表达的诱导可以通过对蛋白激酶 C (PKC) 消耗细胞和显性失活 Raf-1 突变体的不同敏感性来区分。因此，PKC 耗竭和显性失活 Raf-1 突变体能够区分 v-Src 激活的两种细胞内信号传导机制。这两种 v-Src 诱导的细胞内信号都对 Ha-Ras 的显性失活突变体敏感。这些数据表明 Ha-Ras 的功能是协调调节 v-Src 激活的多种细胞内信号传导机制。
v-Src activates promoters under the control of 12-O-tetradecanoylphorbol-13-acetate (TPA) response elements (TREs) and serum response elements (SREs) via two distinguishable intracellular signaling mechanisms. The induction of TRE- and SRE-mediated gene expression by v-Src could be distinguished by a differential sensitivity to depleting cells of protein kinase C (PKC) and to a dominant negative Raf-1 mutant. Thus, PKC depletion and the dominant negative Raf-1 mutant were able to distinguish two intracellular signaling mechanisms activated by v-Src. Both of these v-Src-induced intracellular signals were sensitive to a dominant negative mutant of Ha-Ras. These data suggest that Ha-Ras functions to coordinately regulate multiple intracellular signaling mechanisms activated by v-Src.